Culturing immobilized plant cells for the TUBUL space experiments on the DELTA and 12S Missions
| العنوان: | Culturing immobilized plant cells for the TUBUL space experiments on the DELTA and 12S Missions |
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| المؤلفون: | Jan W. Vos, Björn J. Sieberer, Anne Mie C. Emons |
| المصدر: | Microgravity-Science and Technology, 19(5-6), 191-195 Microgravity-Science and Technology 19 (2007) 5-6 |
| بيانات النشر: | Springer Science and Business Media LLC, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Chromatography, EPS-1, Cell division, Chemistry, Applied Mathematics, Microtubule cytoskeleton, General Engineering, General Physics and Astronomy, Nylon mesh, Laboratorium voor Celbiologie, Nanotechnology, Liquid medium, Plant cell, microtubules, On board, Laboratory of Cell Biology, chemistry.chemical_compound, Modeling and Simulation, Agarose, Stationary growth |
| الوصف: | For the TUBUL experiments during the DELTA mission in April 2004 and 12S mission in March/April 2006 on board the Soyuz capsule and the International Space Station we developed a method to culture and chemically fix plant suspension culture cells. The aim of the ten day experiment was to investigate the effect of microgravity on single plant cells. Fully automated experiment cassettes (Plunger Box Units) were developed by Centre for Concepts in Mechatronics (Nuenen, the Netherlands). Tobacco BY- 2 cells were immobilized in a semi- solid agarose matrix that was reinforced by a nylon mesh. This assembly allowed liquid medium refreshment, oxygen supply and chemical fixation, including a post- fixative wash. The method was optimized for post- flight analysis of cell structure,shape and size, cell division, and the microtubule cytoskeleton. The viability of cells in the agarose matrix was similar to cells grown in liquid medium under laboratory conditions, only the stationary growth phase was reached six days later |
| وصف الملف: | application/octet-stream; application/pdf |
| تدمد: | 1875-0494 0938-0108 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c928d016ba92415b34ffe3f6bc215958Test https://doi.org/10.1007/bf02919480Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....c928d016ba92415b34ffe3f6bc215958 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18750494 09380108 |
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