Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata
| العنوان: | Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata |
|---|---|
| المؤلفون: | Francisco M. Pinto, Idaira Dorta, Sara Marrero-Hernández, Delia Báez, Ayoze González-Santana, A. R. Bello, Mariano Hernández, Teresa A. Almeida, Luz Candenas |
| المساهمون: | Ministerio de Economía y Competitividad (España), European Commission, Gobierno de Canarias |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, medicine.medical_specialty, animal structures, NK1 receptor short isoform, Neurokinin A, Blotting, Western, Substance P, Biology, Real-Time Polymerase Chain Reaction, digestive system, complex mixtures, Leiomyomas, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, TAC1, Internal medicine, Tachykinins, Tachykinin receptor 1, medicine, Biomarkers, Tumor, Humans, RNA, Messenger, Receptor, Uterine leiomyoma, Leiomyoma, Reverse Transcriptase Polymerase Chain Reaction, musculoskeletal, neural, and ocular physiology, Myometrium, Obstetrics and Gynecology, Receptors, Neurokinin-2, Middle Aged, Receptors, Neurokinin-1, Immunohistochemistry, Tachykinin receptors, female genital diseases and pregnancy complications, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Endocrinology, Reproductive Medicine, chemistry, 030220 oncology & carcinogenesis, Uterine Neoplasms, Female, Tachykinin receptor |
| الوصف: | [Objective] To study the expression levels of tachykinins and tachykinin receptors in uterine leiomyomas and matched myometrium. [Design] Laboratory study. [Setting] University research laboratories and academic hospital. [Patient(s)] Women undergoing hysterectomy for symptomatic leiomyomas. [Intervention(s)] Quantitative polymerase chain reaction, immunohistochemistry and Western blot. [Main Outcome Measure(s)] Expression and tissue immunostaining of substance P, neurokinin A, hemokinin-1, neurokinin 1 receptor full-length (NK1R-Fl) and truncated (NK1R-Tr) isoforms, and neurokinin 2 receptor (NK2R) in paired samples of leiomyoma and adjacent normal myometrium. [Result(s)] TAC1 messenger RNA (mRNA) was significantly up-regulated in leiomyomas, whereas intense immunoreaction for the three peptides was particularly abundant in connective tissue cells. Differential regulation of TACR1 mRNA was observed, and at the protein level there was a significant increased expression of NK1R short isoform (NK1R-Tr). TACR2 mRNA was significantly up-regulated in leiomyomas, although levels of NK2R protein were similar in normal and tumor cells. [Conclusion(s)] These and our previous data demonstrate that the whole tachykinin system is differentially regulated in leiomyomas. The increased expression of NK1R-Tr might stimulate leiomyoma growth in a similar way to that observed in other steroid-dependent tumors. Supported in part by research grants from the Agencia Canaria de Innovación y Sociedad de la Información, ACIISI, (PI 2007/001), and from Ministerio de Economía y Competitividad (CTQ2011-25564 and RTC-2014-1431-1), Spain, in both cases with joint financing by FEDER funds from the European Union. |
| تدمد: | 1556-5653 2011-2556 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8e7ae60c47b9d0b43e214681d8545d4Test https://pubmed.ncbi.nlm.nih.gov/27456549Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c8e7ae60c47b9d0b43e214681d8545d4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15565653 20112556 |
|---|