A New Immobilized NAD+ Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme
| العنوان: | A New Immobilized NAD+ Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme |
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| المؤلفون: | Klaus Mosbach, Margareta Lindberg, Per-Olof Larsson |
| المصدر: | European Journal of Biochemistry. 40:187-193 |
| بيانات النشر: | Wiley, 1973. |
| سنة النشر: | 1973 |
| مصطلحات موضوعية: | Alkylation, Chemical Phenomena, Stereochemistry, Coenzymes, Iodoacetates, Dehydrogenase, Diamines, Biochemistry, Malate dehydrogenase, Chromatography, Affinity, Cofactor, chemistry.chemical_compound, Malate Dehydrogenase, Polysaccharides, Lactate dehydrogenase, Acetamides, Cyanogen Bromide, Phosphoglycerate dehydrogenase, Alcohol dehydrogenase, Chromatography, L-Lactate Dehydrogenase, biology, Chemistry, NAD, Alcohol Oxidoreductases, Glycerol-3-phosphate dehydrogenase, biology.protein, Spectrophotometry, Ultraviolet, NAD+ kinase |
| الوصف: | Alkylation of NAD+ with iodoacetic acid followed by alkaline rearrangement gave N6-carboxymethyl-NAD+. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD+- N6-[N-(6-aminohexyl)-acetamide]. The coenzymic activity of the two derivatives was tested with malate dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The efficiency relative to unsubstituted NAD+ was in the range of 50–100%. NAD+-N6-[N-(6-aminohexyl)-acetamide] was attached to Sepharose 4B by the cyanogen bromide method. The immobilized NAD+ analogue thus obtained, exhibited cofactor activity when tested in a recycling three-enzyme system (malate dehydrogenease-citrate synthase-lactate dehydrogenase). The immobilized NAD+ analogue proved to be and effective ligand in affinity chromatography. Thus a mixture of albumin, alcohol dehydrogenase and lactate dehydrogenase was resolved with good recovery. The enzymes were eluted with NAD+ and NADH, respectively. |
| تدمد: | 1432-1033 0014-2956 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8751219f1aec89189f2522ac2d3d4daTest https://doi.org/10.1111/j.1432-1033.1973.tb03184.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c8751219f1aec89189f2522ac2d3d4da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
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