Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice
| العنوان: | Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice |
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| المؤلفون: | Susanne Bartsch, Kai Flachsbarth, Boris Fehse, Mahmoud Bassal, Udo Bartsch, Simon Dulz, Kristoffer Riecken, Stefanie Schlichting |
| المصدر: | Cells, Vol 9, Iss 2082, p 2082 (2020) Cells Volume 9 Issue 9 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Retinal Ganglion Cells, medicine.medical_specialty, genetic structures, medicine.medical_treatment, lentiviral vectors, Ciliary neurotrophic factor, Retinal ganglion, Article, optic nerve, Lesion, 03 medical and health sciences, Mice, 0302 clinical medicine, Neurotrophic factors, Internal medicine, Glial cell line-derived neurotrophic factor, CNTF, Medicine, Animals, Ciliary Neurotrophic Factor, Glial Cell Line-Derived Neurotrophic Factor, lcsh:QH301-705.5, neural stem cells, biology, Cell Death, business.industry, General Medicine, GDNF, Neural stem cell, eye diseases, axotomy, Disease Models, Animal, 030104 developmental biology, Endocrinology, nervous system, lcsh:Biology (General), regeneration, Intravitreal Injections, biology.protein, Optic nerve, neuroprotection, sense organs, Axotomy, medicine.symptom, business, 030217 neurology & neurosurgery |
| الوصف: | We have recently demonstrated that neural stem cell-based intravitreal co-administration of glial cell line-derived neurotrophic factor (GDNF) and ciliary neurotrophic factor (CNTF) confers profound protection to injured retinal ganglion cells (RGCs) in a mouse optic nerve crush model, resulting in the survival of ~38% RGCs two months after the nerve lesion. Here, we analyzed whether this neuroprotective effect is long-lasting and studied the impact of the pronounced RGC rescue on axonal regeneration. To this aim, we co-injected a GDNF- and a CNTF-overexpressing neural stem cell line into the vitreous cavity of adult mice one day after an optic nerve crush and determined the number of surviving RGCs 4, 6 and 8 months after the lesion. Remarkably, we found no significant decrease in the number of surviving RGCs between the successive analysis time points, indicating that the combined administration of GDNF and CNTF conferred lifelong protection to injured RGCs. While the simultaneous administration of GDNF and CNTF stimulated pronounced intraretinal axon growth when compared to retinas treated with either factor alone, numbers of regenerating axons in the distal optic nerve stumps were similar in animals co-treated with both factors and animals treated with CNTF only. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8691c3ec94e2b87ae48f3f5472d99fbTest https://www.mdpi.com/2073-4409/9/9/2082Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c8691c3ec94e2b87ae48f3f5472d99fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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