Correlation between LTR point mutations and proviral load levels among Human T cell Lymphotropic Virus type 1 (HTLV-1) asymptomatic carriers
| العنوان: | Correlation between LTR point mutations and proviral load levels among Human T cell Lymphotropic Virus type 1 (HTLV-1) asymptomatic carriers |
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| المؤلفون: | Ester Cerdeira Sabino, Sabri Saeed Sanabani, Antonio C Da-Costa, Walter Kleine Neto, Youko Nukui, Ana Carolina Soares de Oliveira, Vanessa Pouza Martinez |
| المصدر: | Virology Journal, Vol 8, Iss 1, p 535 (2011) Virology Journal |
| بيانات النشر: | BMC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Adult, Male, Mutant, Biology, Response Elements, Virus Replication, Genetic analysis, lcsh:Infectious and parasitic diseases, Young Adult, Proviruses, Virology, Humans, Point Mutation, lcsh:RC109-216, Human T cell lymphotropic virus type 1, Aged, Aged, 80 and over, Human T-lymphotropic virus 1, Point mutation, Research, Genes, pX, Terminal Repeat Sequences, Gene Products, tax, Middle Aged, Viral Load, HTLV-I Infections, Long terminal repeat, Infectious Diseases, Cross-Sectional Studies, Viral replication, Carrier State, Female, Viral load, Asymptomatic carrier |
| الوصف: | Background In vitro studies have demonstrated that deletions and point mutations introduced into each 21 bp imperfect repeat of Tax-responsive element (TRE) of the genuine human T-cell leukemia virus type I (HTLV-1) viral promoter abolishes Tax induction. Given these data, we hypothesized that similar mutations may affect the proliferation of HTLV-1i nfected cells and alter the proviral load (PvL). To test this hypothesis, we conducted a cross-sectional genetic analysis to compare the near-complete LTR nucleotide sequences that cover the TRE1 region in a sample of HTLV-1 asymptomatic carriers with different PvL burden. Methods A total of 94 asymptomatic HTLV-1 carriers with both sequence from the 5' long terminal repeat (LTR) and a PvL for Tax DNA measured using a sensitive SYBR Green real-time PCR were studied. The 94 subjects were divided into three groups based on PvL measurement: 31 low, 29 intermediate, and 34 high. In addition, each group was compared based on sex, age, and viral genotypes. In another analysis, the median PvLs between individuals infected with mutant and wild-type viruses were compared. Results Using a categorical analysis, a G232A substitution, located in domain A of the TRE-1 motif, was detected in 38.7% (12/31), 27.5% (8/29), and 61.8% (21/34) of subjects with low, intermediate, or high PvLs, respectively. A significant difference in the detection of this mutation was found between subjects with a high or low PvL and between those with a high or intermediate PvL (both p < 0.05), but not between subjects with a low or intermediate PvL (p > 0.05). This result was confirmed by a non-parametric analysis that showed strong evidence for higher PvLs among HTLV-1 positive individuals with the G232A mutation than those without this mutation (p < 0.03). No significant difference was found between the groups in relation to age, sex or viral subtypes (p > 0. 05). Conclusions The data described here show that changes in domain A of the HTLV-1 TRE-1 motif resulting in the G232A mutation may increase HTLV-1 replication in a majority of infected subjects. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5fd98baea5acfd07cc662201a556a1aTest http://www.virologyj.com/content/8/1/535Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c5fd98baea5acfd07cc662201a556a1a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |