Bone morphogenetic protein 4 promotes the survival and preserves the structure of flow-sorted Bhlhb5+ cochlear spiral ganglion neurons in vitro
| العنوان: | Bone morphogenetic protein 4 promotes the survival and preserves the structure of flow-sorted Bhlhb5+ cochlear spiral ganglion neurons in vitro |
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| المؤلفون: | Shan Sun, Xia Gao, Irum-us Islam, Jieyu Qi, Qiaojun Fang, Huawei Li, Shasha Zhang, Renjie Chai, Chuanyin Xuan, Muhammad Waqas, Haibo Shi, Xiaoli Zhang, Mingliang Tang |
| المصدر: | Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017) Scientific Reports |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, animal structures, Neurite, Cell Survival, Science, medicine.medical_treatment, Growth Cones, Neuronal Outgrowth, Population, Apoptosis, Mice, Transgenic, Bone Morphogenetic Protein 4, In Vitro Techniques, Biology, Article, 03 medical and health sciences, 0302 clinical medicine, Basic Helix-Loop-Helix Transcription Factors, otorhinolaryngologic diseases, medicine, Animals, education, Growth cone, Cells, Cultured, Spiral ganglion, Neurons, education.field_of_study, Multidisciplinary, Growth factor, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Bone morphogenetic protein 4, Synapses, embryonic structures, Medicine, sense organs, Spiral Ganglion, Filopodia, 030217 neurology & neurosurgery, Explant culture |
| الوصف: | SGNs are the primary auditory neurons, and damage or loss of SGNs leads to sensorineural hearing loss. BMP4 is a growth factor that belongs to the TGF-β superfamily and has been shown to play a key role during development, but little is known about its effect on postnatal cochlear SGNs in mice. In this study, we used the P3 Bhlhb5-cre/tdTomato transgenic mouse model and FACS to isolate a pure population of Bhlhb5+ SGNs. We found that BMP4 significantly promoted SGN survival after 7 days of culture. We observed fewer apoptotic cells and decreased expression of pro-apoptotic marker genes after BMP4 treatment. We also found that BMP4 promoted monopolar neurite outgrowth of isolated SGNs, and high concentrations of BMP4 preserved the number and the length of neurites in the explant culture of the modiolus harboring the SGNs. We showed that high concentration of BMP4 enhanced neurite growth as determined by the higher average number of filopodia and the larger area of the growth cone. Finally, we found that high concentrations of BMP4 significantly elevated the synapse density of SGNs in explant culture. Thus, our findings suggest that BMP4 has the potential to promote the survival and preserve the structure of SGNs. |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5538d5b920802c2d5c80e5a5e52d801Test https://doi.org/10.1038/s41598-017-03810-wTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c5538d5b920802c2d5c80e5a5e52d801 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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