MicroRNA‑708 inhibits the proliferation and invasion of osteosarcoma cells by directly targeting ZEB1
| العنوان: | MicroRNA‑708 inhibits the proliferation and invasion of osteosarcoma cells by directly targeting ZEB1 |
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| المؤلفون: | Deng Xiang, Yanshui Lin, Jun He |
| المصدر: | Molecular Medicine Reports. |
| بيانات النشر: | Spandidos Publications, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Cancer Research, Cell, Down-Regulation, Biology, Biochemistry, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Cell Line, Tumor, microRNA, Genetics, medicine, Humans, 3' Untranslated Regions, Molecular Biology, Cell Proliferation, Osteosarcoma, Base Sequence, Oncogene, Cell growth, Antagomirs, Zinc Finger E-box-Binding Homeobox 1, Middle Aged, Cell cycle, medicine.disease, Molecular medicine, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Oncology, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Molecular Medicine, Female, Sequence Alignment |
| الوصف: | Numerous microRNAs (miRNAs) have been identified as aberrantly expressed in osteosarcoma (OS). miRNAs serve important roles in the pathogenesis of OS as oncogenes or tumor suppressors. Recent studies revealed that miR‑708‑5p (miR‑708) was dysregulated in various types of human cancer; however, its roles and underlying molecular mechanisms in OS remain unknown. Therefore, the present study aimed to determine miR‑708 expression in OS, investigate the roles of miR‑708 in the progression of OS and reveal the potential mechanisms involved. It was demonstrated using reverse transcription‑polymerase chain reaction that miR‑708 was downregulated in OS tissues and cell lines. Cell Counting Kit‑8 and Transwell assays revealed that miR‑708 overexpression suppressed the proliferation and invasion of OS cells in vitro. Additionally, zinc finger E‑box binding homeobox 1 (ZEB1) was validated as a direct target gene of miR‑708 in OS cells. ZEB1 was upregulated in OS tissues; elevated ZEB1 expression was negatively correlated with the levels of miR‑708 expression. Rescue experiments indicated that ZEB1 reintroduction significantly counteracted the inhibitory effects of miR‑708 overexpression on the proliferation and invasion of OS cells. The findings may improve understanding of the roles of miR‑708 in the development of OS, and suggest that miR‑708 may be a potential novel therapeutic target in the treatment of patients with this disease. |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c51caad816f0ac7abb4c67c6ae12f92cTest https://doi.org/10.3892/mmr.2019.10013Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c51caad816f0ac7abb4c67c6ae12f92c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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