Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso
| العنوان: | Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso |
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| المؤلفون: | Liz Karen Taho, R. Serge Yerbanga, Anyirékun Fabrice Somé, Cathérine Neya, Frederic Nikiema, Jean-Bosco Ouédraogo, Issaka Zongo, Thomas Bazié |
| المصدر: | Parasites & Vectors, Vol 11, Iss 1, Pp 1-8 (2018) Parasites & Vectors |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Adolescent, 030231 tropical medicine, 030106 microbiology, Plasmodium falciparum, Protozoan Proteins, Antigens, Protozoan, Polymerase Chain Reaction, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Gene Frequency, parasitic diseases, Burkina Faso, medicine, Humans, lcsh:RC109-216, Allele, Merozoite surface protein, Malaria, Falciparum, Child, Allele frequency, Genotyping, Alleles, Merozoite Surface Protein 1, Genetic diversity, biology, Coinfection, Research, Genetic Variation, Infant, Middle Aged, medicine.disease, biology.organism_classification, Virology, Infectious Diseases, msp2, Child, Preschool, msp1, Parasitology, Female, Nested polymerase chain reaction, Malaria |
| الوصف: | Background In Burkina Faso, malaria remains the overall leading cause of morbidity and mortality accounting for 35.12% of consultations, 40.83% of hospitalizations and 37.5% of deaths. Genotyping of malaria parasite populations remains an important tool to determine the types and number of parasite clones in an infection. The present study aimed to evaluate the merozoite surface protein 1 (msp1) and merozoite surface protein 2 (msp2) genetic diversity and allele frequencies in Bobo-Dioulasso, Burkina Faso. Method Dried blood spots (DBS) were collected at baseline from patients with uncomplicated malaria in urban health centers in Bobo-Dioulasso. Parasite DNA was extracted using chelex-100 and species were identified using nested PCR. Plamodium falciparum msp1 and msp2 genes were amplified by nested polymerase chain reaction (PCR) and PCR products were analyzed by electrophoresis on a 2.5% agarose gel. Alleles were categorized according to their molecular weight. Results A total of 228 blood samples were analyzed out of which 227 (99.9%) were confirmed as P. falciparum-positive and one sample classified as mixed infection for P. malaria and P. falciparum. In msp1, the K1 allelic family was predominant with 77.4% (162/209) followed respectively by the MAD20 allelic family with 41.3% and R033 allelic family with 36%. In msp2, the 3D7 allelic family was the most frequently detected with 93.1 % compared to FC27 with 41.3%. Twenty-one different alleles were observed in msp1 with 9 alleles for K1, 8 alleles for MAD20 and 4 alleles for R033. In msp2, 25 individual alleles were detected with 10 alleles for FC27 and 15 alleles for 3D7. The mean multiplicity of falciparum infection was 1.95 with respectively 1.8 (1.76–1.83) and 2.1 (2.03–2.16) for msp1 and msp2 (P = 0.01). Conclusions Our study showed high genetic diversity and allelic frequencies of msp1 and msp2 in Plasmodium falciparum isolates from symptomatic malaria patients in Bobo-Dioulasso. |
| اللغة: | English |
| تدمد: | 1756-3305 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c35af200c2e70c644e36ff93611e8712Test http://link.springer.com/article/10.1186/s13071-018-2895-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c35af200c2e70c644e36ff93611e8712 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17563305 |
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