Relationship between expression of PD-L1 and PD-L2 on esophageal squamous cell carcinoma and the antitumor effects of CD8+ T cells
| العنوان: | Relationship between expression of PD-L1 and PD-L2 on esophageal squamous cell carcinoma and the antitumor effects of CD8+ T cells |
|---|---|
| المؤلفون: | Yan Zheng, Wei Wang, Yin Li, Yongkui Yu, Xianben Liu, Changsen Leng, Yingying Cui, Zongfei Wang, Xionghuai Hua, Jun Zhang, Jun Ma, Haibo Sun, Junwei Zhu, Qiuming Wang, Jianjun Qin, Haomiao Li |
| المصدر: | Oncology Reports. 35:699-708 |
| بيانات النشر: | Spandidos Publications, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Cancer Research, Esophageal Neoplasms, Programmed Cell Death 1 Ligand 2 Protein, medicine.medical_treatment, Cell Separation, Kaplan-Meier Estimate, CD8-Positive T-Lymphocytes, Real-Time Polymerase Chain Reaction, Transfection, B7-H1 Antigen, 03 medical and health sciences, Lymphocytes, Tumor-Infiltrating, 0302 clinical medicine, Immune system, PD-L1, medicine, Humans, Cytotoxic T cell, Aged, Aged, 80 and over, biology, General Medicine, Immunotherapy, Middle Aged, Cell cycle, Flow Cytometry, Prognosis, Immunohistochemistry, Coculture Techniques, Cell biology, 030104 developmental biology, Oncology, Apoptosis, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, biology.protein, Cancer research, Female, Tumor Escape, Esophageal Squamous Cell Carcinoma, CD8 |
| الوصف: | The programmed death-1 (PD-1)/programmed death-ligands (PD-Ls) signal pathway has been implicated as a potential immune escape mechanism in several human cancers. However, the studies of PD‑1/PD‑Ls pathway in esophageal squamous cell carcinoma (ECSS) are not yet sufficient. The current study investigated the expression of PD‑L1, PD‑L2 and PD‑1 in ESCC tissues. The correlations between the expression of these proteins and clinical histopathological parameters were analyzed. Then the stable transfected Ec109 cell lines overexpressing PD‑L1/PD‑L2 were established by plasmid transfection successfully. Ec109 and CD8+ T cells were co‑cultured to analyze the effects of PD‑1/PD‑Ls signal pathway on the function of CD8+ T cells including proliferation, apoptosis and interferon‑γ production. We found that PD‑L1-positive patients had significantly poorer prognosis than the negative patients, while their prognosis was not related to PD‑L2 expression. The count of PD‑1+ TILs (tumor‑infiltrating lymphocytes) was negatively correlated with both PD‑L1 and PD‑L2 expression. In functional studies, we found that PD‑1/PD‑Ls signal pathway was able to downregulate the function of CD8+ T lymphocyte and its function could be restored by blocking the signal pathway. This indicates that PD‑1/PD‑Ls may prevent effective antitumor immunity, which provides important evidence to delineate the cellular immune deficiency mechanism in ESCC. Therefore, PD-1/PD-Ls are predicted to become novel targets for ESCC immunotherapy. |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2a5fea601fe63d0e8c980c05b31b6e0Test https://doi.org/10.3892/or.2015.4435Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c2a5fea601fe63d0e8c980c05b31b6e0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
|---|