Muscarinic acetylcholine receptor M1 mediates prostate cancer cell migration and invasion through hedgehog signaling
| العنوان: | Muscarinic acetylcholine receptor M1 mediates prostate cancer cell migration and invasion through hedgehog signaling |
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| المؤلفون: | Qing-Qing Yin, Chen Xu, Lin-Hui Xu, Mi Zhang |
| المصدر: | Asian Journal of Andrology, Vol 20, Iss 6, Pp 608-614 (2018) Asian Journal of Andrology |
| بيانات النشر: | Medknow, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Patched, Urology, Muscarinic Antagonists, Muscarinic Agonists, lcsh:RC870-923, Zinc Finger Protein GLI1, muscarinic acetylcholine receptor M1 (CHRM1), 03 medical and health sciences, 0302 clinical medicine, Cell Movement, GLI1, Muscarinic acetylcholine receptor, LNCaP, medicine, Humans, metastasis, Hedgehog Proteins, Sonic hedgehog, Cell Proliferation, biology, Receptor, Muscarinic M1, Prostatic Neoplasms, Pirenzepine, General Medicine, Muscarinic acetylcholine receptor M1, prostate cancer, lcsh:Diseases of the genitourinary system. Urology, hedgehog signaling, Hedgehog signaling pathway, Patched-1 Receptor, 030104 developmental biology, 030220 oncology & carcinogenesis, biology.protein, Cancer research, Carbachol, Original Article, medicine.drug |
| الوصف: | The autonomic nervous system contributes to prostate cancer proliferation and metastasis. However, the exact molecular mechanism remains unclear. In this study, muscarinic acetylcholine receptor M1 (CHRM1) expression was measured via immunohistochemical analysis in human prostate cancer tissue array slides. PC-3, LNCaP, and A549 cells were treated with pirenzepine or carbachol, and the cell migration and invasion abilities were evaluated. Western blotting and quantitative real-time PCR were performed to measure GLI family zinc finger 1 (GLI1), patched 1 (PTCH1), and sonic hedgehog (SHH) expression levels. High expression of CHRM1 was found in early-stage human prostate cancer tissues. In addition, the selective CHRM1 antagonist pirenzepine inhibited PC-3, LNCaP, and A549 cell migration and invasion, but the agonist carbachol promoted the migration and invasion of these three cell lines. Muscarinic signaling can be relayed by hedgehog signaling. These data show that CHRM1 is involved in the regulation of prostate cancer migration and invasion through the hedgehog signaling pathway. |
| تدمد: | 1008-682X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c29b809bfa080007420247ea8c1d79f4Test https://doi.org/10.4103/aja.aja_55_18Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c29b809bfa080007420247ea8c1d79f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1008682X |
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