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Profiling of circulating microRNAs reveals common microRNAs linked to type 2 diabetes that change with insulin sensitization

التفاصيل البيبلوغرافية
العنوان: Profiling of circulating microRNAs reveals common microRNAs linked to type 2 diabetes that change with insulin sensitization
المؤلفون: Sophie Rome, Oscar Rovira, José María Moreno-Navarrete, Eduardo Esteve, Jennifer Rieusset, Gemma Xifra, Francisco J. Ortega, José Manuel Fernández-Real, Josep M. Mercader, Ester Guerra, Wifredo Ricart, Marek Straczkowski, Monika Karczewska-Kupczewska, Cristina Martínez
المساهمون: Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Programme Innovative Economy [UDA-POIG.01.03.01-00-128/08], European Union within the European Regional Development Fund, Spanish Ministry of Science and Innovation [FIS 2011-00214], CIBER de la Fisiopatologia de la Obesidad y la Nutricion (CIBERobn), Associacio Catalana de Diabetis, Sara Borrell fellowship, ISCIII, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL)
المصدر: Diabetes Care
Diabetes Care, American Diabetes Association, 2014, 37 (5), pp.1375-1383. ⟨10.2337/dc13-1847⟩
بيانات النشر: HAL CCSD, 2014.
سنة النشر: 2014
مصطلحات موضوعية: Male, endocrine system diseases, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, [SDV]Life Sciences [q-bio], administration & dosage, Pilot Projects, pharmacology"*"Insulin Resistance, Type 2 diabetes, 0302 clinical medicine, Insulin, Sensitization, 0303 health sciences, Heparin, Middle Aged, Metformin, drug therapy, medicine.anatomical_structure, genetics"*"Male"*"Metformin, Female, Type 2, medicine.drug, Adult, medicine.medical_specialty, Adolescent, 030209 endocrinology & metabolism, Adolescent"*"Adult"*"Aged"*"Biological Markers/metabolism"*"Cross-Sectional Studies"*"Diabetes Mellitus, metabolism"*"Middle Aged"*"Pilot Projects"*"Young Adult, 03 medical and health sciences, Young Adult, Insulin resistance, therapeutic use"*"MicroRNAs, Double-Blind Method, blood, Diabetes mellitus, Internal medicine, Internal Medicine, medicine, Humans, Hypoglycemic Agents, Type 2/blood/drug therapy/*genetics"*"Double-Blind Method"*"Female"*"Humans"*"Hypoglycemic Agents/administration & dosage/pharmacology"*"Insulin/administration & dosage/pharmacology"*"Insulin Resistance/*genetics"*"Male"*"Metformin/therapeutic use"*"MicroRNAs/*metabolism"*"Middle Aged"*"Pilot Projects"*"Young Adult, 030304 developmental biology, Aged, Advanced and Specialized Nursing, business.industry, pharmacology"*"Insulin, medicine.disease, Circulating MicroRNA, MicroRNAs, Endocrinology, Cross-Sectional Studies, Diabetes Mellitus, Type 2, genetics"*"Double-Blind Method"*"Female"*"Humans"*"Hypoglycemic Agents, Insulin Resistance, business, Biomarkers
الوصف: OBJECTIVE This study sought to identify the profile of circulating microRNAs (miRNAs) in type 2 diabetes (T2D) and its response to changes in insulin sensitivity. RESEARCH DESIGN AND METHODS The circulating miRNA profile was assessed in a pilot study of 12 men: 6 with normal glucose tolerance (NGT) and 6 T2D patients. The association of 10 circulating miRNAs with T2D was cross-sectionally validated in an extended sample of 45 NGT vs. 48 T2D subjects (65 nonobese and 28 obese men) and longitudinally in 35 T2D patients who were recruited in a randomized, double-blinded, and placebo-controlled 3-month trial of metformin treatment. Circulating miRNAs were also measured in seven healthy volunteers before and after a 6-h hyperinsulinemic-euglycemic clamp and insulin plus intralipid/heparin infusion. RESULTS Cross-sectional studies disclosed a marked increase of miR-140-5p, miR-142-3p, and miR-222 and decreased miR-423-5p, miR-125b, miR-192, miR-195, miR-130b, miR-532-5p, and miR-126 in T2D patients. Multiple linear regression analyses revealed that miR-140-5p and miR-423-5p contributed independently to explain 49.5% (P < 0.0001) of fasting glucose variance after controlling for confounders. A discriminant function of four miRNAs (miR-140-5p, miR-423-5p, miR-195, and miR-126) was specific for T2D with an accuracy of 89.2% (P < 0.0001). Metformin (but not placebo) led to significant changes in circulating miR-192 (49.5%; P = 0.022), miR-140-5p (−15.8%; P = 0.004), and miR-222 (−47.2%; P = 0.03), in parallel to decreased fasting glucose and HbA1c. Furthermore, while insulin infusion during clamp decreased miR-222 (−62%; P = 0.002), the intralipid/heparin mixture increased circulating miR-222 (163%; P = 0.015) and miR-140-5p (67.5%; P = 0.05). CONCLUSIONS This study depicts the close association between variations in circulating miRNAs and T2D and their potential relevance in insulin sensitivity.
اللغة: English
تدمد: 0149-5992
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1a38e0f19ac363323dc70fa330547a2Test
https://hal.archives-ouvertes.fr/hal-01859430Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....c1a38e0f19ac363323dc70fa330547a2
قاعدة البيانات: OpenAIRE
الوصف
تدمد:01495992