Clean version: Electrospun fibrinogen scaffolds from discarded blood for wound healing
| العنوان: | Clean version: Electrospun fibrinogen scaffolds from discarded blood for wound healing |
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| المؤلفون: | Wang Weiyu, Sun Yu, Minxian Ma, Ye Chuan, Long Yang, Qin Liu, Zhixu He, Qiang Zou, Tao Lu |
| المصدر: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 109:1145-1155 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Scaffold, Materials science, Biomedical Engineering, 02 engineering and technology, Mesenchymal Stem Cell Transplantation, 010402 general chemistry, 01 natural sciences, Rats, Sprague-Dawley, Biomaterials, Extracellular matrix, Tissue engineering, Animals, Humans, Viability assay, Wound Healing, Tissue Scaffolds, Mesenchymal stem cell, Fibrinogen, Mesenchymal Stem Cells, Cells, Immobilized, 021001 nanoscience & nanotechnology, Electrospinning, Rats, 0104 chemical sciences, Blood, Hemostasis, Heterografts, 0210 nano-technology, Wound healing, Biomedical engineering |
| الوصف: | Immediate reutilization of discarded blood from surgery has not received much attention, leading to the waste of a large amount of autologous blood. We used a concentration gradient and high-voltage electrospinning technology to immediately prepare a scaffold material with high biological activity but without immunogenicity from autologous waste blood collected during surgery. Here, we fabricated and characterized a 90 mg/mL group, 110 mg/mL group, and 130 mg/mL group of fibrinogen (FBG) scaffolds. Analyses revealed that the FBG scaffolds had good film-forming properties and a clear fiber structure. in vitro cell viability experiments confirmed that the cells showed an increasing trend with increasing FBG concentrations. The cells grew well in the scaffold material and secreted more cell matrix. When human bone mesenchymal stem cells (hBMSCs) were cocultured with the scaffold material, the hBMSCs expressed osteogenic and chondrogenic biomarkers. The cellular scaffold complexes from the 3 groups were implanted in four full-thickness round wounds (Φ12 mm) on the dorsal back of each rat, the 130 mg/mL group showed a 90% reduction in wound size and the data compared to other groups were better at 14 day. These results suggest that electrospinning technology-based FBG scaffold materials derived from autologous waste blood may become an ideal tissue engineering scaffold and can be immediately used for autologous hemostasis, anti-adhesion films, and wound dressing in surgery. |
| تدمد: | 1552-4981 1552-4973 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfd929d20d7592b4bcb5168fe70a8838Test https://doi.org/10.1002/jbm.b.34777Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....bfd929d20d7592b4bcb5168fe70a8838 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15524981 15524973 |
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