Angiotensin 1–7 increases fiber cross-sectional area and force in juvenile mouse skeletal muscle
| العنوان: | Angiotensin 1–7 increases fiber cross-sectional area and force in juvenile mouse skeletal muscle |
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| المؤلفون: | Hind Albadrani, Tarek Ammar, Rashida Rajgara, Michael Bader, Nadine Wiper-Bergeron, Jean-Marc Renaud |
| المصدر: | American Journal of Physiology-Cell Physiology. 323:C1681-C1696 |
| بيانات النشر: | American Physiological Society, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mice, Physiology, Mice, Inbred mdx, Animals, Cell Biology, Angiotensin I, Muscle, Skeletal, Peptide Fragments |
| الوصف: | Recent studies reported that in skeletal muscle angiotensin 1–7 (Ang 1–7), via its receptor Mas (MasR), prevents the atrophy induced by angiotensin II and by cast immobilization; it also improves muscle integrity and function in the mdx mouse, a muscular dystrophy model. The objectives of this study were to document 1) the extent of the Ang 1–7’s hypertrophic effect in terms of muscle mass and muscle fiber cross-sectional area (CSA), 2) how Ang 1–7 affects muscle contractile function in terms of twitch and tetanic force, force-frequency relationship, and 3) whether the effect involves MasR. Wild-type and MasR-deficient [Mas receptor knockout mouse model ( MasR−/−)] mice were treated with Ang 1–7 (100 ng/kg body wt·min using an osmotic pump) for 4 or 16 wk. Ang 1–7 significantly increased skeletal muscle/body weight ratio of soleus, tibialis, and gastrocnemius, but not of extensor digitorum longus (EDL). It significantly increased fiber cross-sectional area in the order of type I > IIA > IIB. In EDL and soleus muscles, it significantly increased twitch and tetanic force while causing a shift in the force-frequency relationship toward lower stimulation frequencies. It had no effect on fiber type composition. None of the Ang 1–7 effects observed in wild-type mice were observed in MasR−/− muscles. It caused a transient increase in phosphorylated protein kinase B (Akt) and 4EBP proteins while having no effect on S6 phosphorylation, MuRF-1, and atrogin-1 and a decrease in PAX7 expression in satellite cells. This is the first study demonstrating the hypertrophic effects of Ang 1–7 in normal muscle acting via its MasR. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf2c6d99855f23b8c21a44f653ffa3cfTest https://doi.org/10.1152/ajpcell.00271.2021Test |
| رقم الانضمام: | edsair.doi.dedup.....bf2c6d99855f23b8c21a44f653ffa3cf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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