التفاصيل البيبلوغرافية
العنوان:
Long term increased expression of the short form 1b prolactin receptor in PC-3 human prostate cancer cells decreases cell growth and migration, and causes multiple changes in gene expression consistent with reduced invasive capacity
المؤلفون:
Ameae M. Walker , Kuang-tzu Huang
المصدر:
The Prostate . 70(1)
سنة النشر:
2009
مصطلحات موضوعية:
Male , medicine.medical_specialty , Time Factors , Receptors, Prolactin , Urology , Basic fibroblast growth factor , Down-Regulation , Biology , Article , chemistry.chemical_compound , Internal medicine , Cell Line, Tumor , medicine , Humans , Protein Isoforms , Neoplasm Invasiveness , Receptor , Cell growth , Prolactin receptor , Prostatic Neoplasms , Cell migration , Growth Inhibitors , Cell biology , Urokinase receptor , Gene Expression Regulation, Neoplastic , Endocrinology , Oncology , chemistry , Cell culture , Cancer cell , Cell Migration Inhibition
الوصف:
BACKGROUND We have shown that treatment of human prostate cancer cells with the selective prolactin (PRL) receptor modulator, S179D PRL, inhibits growth in vitro, and the initiation and growth of xenografts in vivo. S179D PRL treatment also upregulates expression of the short form 1b (SF1b) PRL receptor, activation of which upregulates expression of the cell cycle-regulating protein, p21. METHODS We examined the consequences of long term increased expression and activation of SF1b, at levels comparable to those resulting from treatment with S179D PRL, by creating PC-3-derived stable cell lines expressing a constitutively active form of SF1b, ΔS2 SF1b. RESULTS Increased expression of ΔS2 SF1b decreased growth and migration of the cells. This was accompanied by an increase in cell-matrix interactions, and cell–cell aggregation when cells were plated on basement membrane components. Real-time PCR evaluated the expression of genes related to invasive capacity. Of particular interest was decreased expression of the protease, urokinase-type plaminogen activator, and its receptor, uPAR, and increased expression of its inhibitors, PAI-1 and 2. Also decreased in cells with increased expression of ΔS2 SF1b was expression of basic fibroblast growth factor and vascular endothelial growth factor. CONCLUSION We conclude that at least part of the beneficial effects of S179D PRL is the result of increased expression of SF1b, and that the effects of increased expression and activation of SF1b continue to be of potential benefit in the long term. Prostate 70: 37–47, 2010. © 2009 Wiley-Liss, Inc.
تدمد:
1097-0045
الوصول الحر:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be96eee84c9ef1a06a9e43469dac28e6Test https://pubmed.ncbi.nlm.nih.gov/19739126Test
حقوق:
OPEN
رقم الانضمام:
edsair.doi.dedup.....be96eee84c9ef1a06a9e43469dac28e6
قاعدة البيانات:
OpenAIRE
