Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions
| العنوان: | Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions |
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| المؤلفون: | Benjamin T. Goult, York-Christoph Ammon, Anna Akhmanova, Albert J. R. Heck, A. F. Maarten Altelaar, Harm Post, Rosemarie E. Gough, Dieudonnée van de Willige, Benjamin P. Bouchet, Guillaume Jacquemet |
| المصدر: | eLife, Vol 5 (2016) eLife Elife, 5:e18124. eLife Sciences Publications, Ltd |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Role of cell adhesions in neural development, Microtubules, Tumor Suppressor Proteins/metabolism, Extracellular matrix, 0302 clinical medicine, focal adhesion, Biology (General), 0303 health sciences, biology, Chemistry, General Neuroscience, Adaptor Proteins, Signal transducing adaptor protein, General Medicine, Biophysics and Structural Biology, Cell biology, Microtubules/metabolism, Medicine, Cortical microtubule, Research Article, Human, microtubule, QH301-705.5, Science, Integrin, macromolecular substances, ta3111, General Biochemistry, Genetics and Molecular Biology, Focal adhesion, 03 medical and health sciences, QH301, Microtubule, Cell Adhesion, Humans, Actin, 030304 developmental biology, Adaptor Proteins, Signal Transducing, Focal Adhesions, General Immunology and Microbiology, Point mutation, talin, Focal Adhesions/metabolism, Signal Transducing, ta1182, KANK, Cell Biology, Cytoskeletal Proteins, HEK293 Cells, 030104 developmental biology, Biophysics, Talin/metabolism, biology.protein, 030217 neurology & neurosurgery, HeLa Cells |
| الوصف: | The cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix plays a crucial role in cell polarity and migration. Microtubules regulate the turnover of adhesion sites, and, in turn, focal adhesions promote the cortical microtubule capture and stabilization in their vicinity, but the underlying mechanism is unknown. Here, we show that cortical microtubule stabilization sites containing CLASPs, KIF21A, LL5β and liprins are recruited to focal adhesions by the adaptor protein KANK1, which directly interacts with the major adhesion component, talin. Structural studies showed that the conserved KN domain in KANK1 binds to the talin rod domain R7. Perturbation of this interaction, including a single point mutation in talin, which disrupts KANK1 binding but not the talin function in adhesion, abrogates the association of microtubule-stabilizing complexes with focal adhesions. We propose that the talin-KANK1 interaction links the two macromolecular assemblies that control cortical attachment of actin fibers and microtubules. DOI: http://dx.doi.org/10.7554/eLife.18124.001Test eLife digest Animal cells are organized into tissues and organs. A scaffold-like framework outside of the cells called the extracellular matrix provides support to the cells and helps to hold them in place. Cells attach to the extracellular matrix via structures called focal adhesions on the cell surface; these structures contain a protein called talin. For a cell to be able to move, the existing focal adhesions must be broken down and new adhesions allowed to form. This process is regulated by the delivery and removal of different materials along fibers called microtubules. Microtubules can usually grow and shrink rapidly, but near focal adhesions they are captured at the surface of the cell and become more stable. However, it is not clear how focal adhesions promote microtubule capture and stability. Bouchet et al. found that a protein called KANK1 binds to the focal adhesion protein talin in human cells grown in a culture dish. This allows KANK1 to recruit microtubules to the cell surface around the focal adhesions by binding to particular proteins that are associated with microtubules. Disrupting the interaction between KANK1 and talin by making small alterations in these two proteins blocked the ability of focal adhesions to capture surrounding microtubules. The next step following on from this work will be to find out whether this process also takes place in the cells within an animal’s body, such as a fly or a mouse. DOI: http://dx.doi.org/10.7554/eLife.18124.002Test |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2050-084X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcf8514e39a2d26f25dff8b8361415afTest https://doi.org/10.7554/eLife.18124Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bcf8514e39a2d26f25dff8b8361415af |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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