Targeting PD-L1 (Programmed death-ligand 1) and inhibiting the expression of IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) affect the proliferation and apoptosis of hypopharyngeal carcinoma cells
| العنوان: | Targeting PD-L1 (Programmed death-ligand 1) and inhibiting the expression of IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) affect the proliferation and apoptosis of hypopharyngeal carcinoma cells |
|---|---|
| المؤلفون: | Jisheng Liu, Xudong Yang |
| المصدر: | Bioengineered article-version (VoR) Version of Record Bioengineered, Vol 12, Iss 1, Pp 7755-7764 (2021) |
| بيانات النشر: | Informa UK Limited, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, proliferation, medicine.medical_treatment, Cell, Mice, Nude, Apoptosis, Bioengineering, Applied Microbiology and Biotechnology, B7-H1 Antigen, Hypopharyngeal Carcinoma, Mice, Cell Line, Tumor, PD-L1, medicine, Animals, Humans, Viability assay, programmed cell death 1 ligand 1, Aged, Cell Proliferation, Aged, 80 and over, Gene knockdown, Hypopharyngeal Neoplasms, biology, Chemistry, Growth factor, RNA-Binding Proteins, insulin-like growth factor 2 mRNA-binding protein 2, General Medicine, Middle Aged, Xenograft Model Antitumor Assays, medicine.anatomical_structure, Insulin-like growth factor 2, Cancer research, biology.protein, hypopharyngeal carcinoma, TP248.13-248.65, Research Article, Research Paper, Biotechnology |
| الوصف: | Programmed cell death-ligand 1 (PD-L1) have been attracting increasing attention in cancer diagnosis and treatment. The insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is involved in the progression of multiple types of cancer. So, the role of IGF2BP2 and PD-L1 in hypopharyngeal carcinoma was assessed. Western blotting and immunochemistry were used to evaluate the expression of IGF2BP2 and PD-1/PD-L1. IGF2BP2 expression was knocked down in FaDu cells, and the effects on cell viability, apoptosis and proliferation were measured. A tumor-bearing nude model of hypopharyngeal carcinoma was constructed to evaluate the effect of a PD-L1 inhibitor and IGF2BP2 knockdown on hypopharyngeal carcinoma in vivo. RNA pull-down assays were used to assess the interaction between IGF2BP2 and PD-L1. The results showed that knockdown of IGF2BP2 inhibited FaDu cell proliferation and promoted apoptosis, as evidenced by the lower cell viability, a higher ratio of TUNEL-positive cells, decreased expression of Bcl-2 and cyclins, and increased expression of cleaved-caspase 3. In vivo, the tumor volume and weight were reduced by both the PD-L1 inhibitor and IGF2BP2 knockdown. Additionally, the interaction between PD-L1 and IGF2BP2 was confirmed. In conclusion, the results in the present study revealed that inhibition of IGF2BP2 might be a potentially relevant method for treating hypopharyngeal carcinoma, and the effects might be mediated via inhibition of the PD-1/PD-L1 axis. |
| تدمد: | 2165-5987 2165-5979 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc5be91fc6698d2c0556a18942a16a3dTest https://doi.org/10.1080/21655979.2021.1983278Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bc5be91fc6698d2c0556a18942a16a3d |
| قاعدة البيانات: | OpenAIRE |
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The insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is involved in the progression of multiple types of cancer. So, the role of IGF2BP2 and PD-L1 in hypopharyngeal carcinoma was assessed. Western blotting and immunochemistry were used to evaluate the expression of IGF2BP2 and PD-1/PD-L1. IGF2BP2 expression was knocked down in FaDu cells, and the effects on cell viability, apoptosis and proliferation were measured. A tumor-bearing nude model of hypopharyngeal carcinoma was constructed to evaluate the effect of a PD-L1 inhibitor and IGF2BP2 knockdown on hypopharyngeal carcinoma in vivo. RNA pull-down assays were used to assess the interaction between IGF2BP2 and PD-L1. The results showed that knockdown of IGF2BP2 inhibited FaDu cell proliferation and promoted apoptosis, as evidenced by the lower cell viability, a higher ratio of TUNEL-positive cells, decreased expression of Bcl-2 and cyclins, and increased expression of cleaved-caspase 3. 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