No Direct Postconditioning Effect of Poloxamer 188 on Mitochondrial Function after Ischemia Reperfusion Injury in Rat Isolated Hearts
| العنوان: | No Direct Postconditioning Effect of Poloxamer 188 on Mitochondrial Function after Ischemia Reperfusion Injury in Rat Isolated Hearts |
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| المؤلفون: | Matthias L. Riess, William J Cleveland, Josephine Eskaf |
| المصدر: | International Journal of Molecular Sciences Volume 22 Issue 9 International Journal of Molecular Sciences, Vol 22, Iss 4879, p 4879 (2021) |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | calcium retention capacity, cardiac arrest, 030204 cardiovascular system & hematology, Pharmacology, Mitochondrion, Mitochondria, Heart, Rats, Sprague-Dawley, chemistry.chemical_compound, 0302 clinical medicine, Adenosine Triphosphate, Medicine, Myocardial infarction, Biology (General), Ischemic Postconditioning, P188, Spectroscopy, ATP synthase, biology, Electron Transport Complex II, General Medicine, oxygen consumption, Computer Science Applications, mitochondria, Chemistry, myocardial infarction, QH301-705.5, cardiac, Cell Respiration, Ischemia, chemistry.chemical_element, Myocardial Reperfusion Injury, Poloxamer, Calcium, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, PEG ratio, Animals, Physical and Theoretical Chemistry, QD1-999, Molecular Biology, ATP synthesis, copolymer, business.industry, Myocardium, Organic Chemistry, medicine.disease, PEG, chemistry, biology.protein, business, Adenosine triphosphate, Reperfusion injury, 030217 neurology & neurosurgery |
| الوصف: | Myocardial infarction is a leading cause for morbidity and mortality worldwide. The only viable treatment for the ischemic insult is timely reperfusion, which further exacerbates myocardial injury. Maintaining mitochondrial function is crucial in preserving cardiomyocyte function in ischemia reperfusion (IR) injury. Poloxamer (P) 188 has been shown to improve cardiac IR injury by improving cellular and mitochondrial function. The aim of this study was to show if P188 postconditioning has direct protective effects on mitochondrial function in the heart. Langendorff prepared rat hearts were subjected to IR injury ex-vivo and reperfused for 10 min with 1 mM P188 vs. vehicle. Cardiac mitochondria were isolated with 1 mM P188 vs. 1 mM polyethylene glycol (PEG) vs. vehicle by differential centrifugation. Mitochondrial function was assessed by adenosine triphosphate synthesis, oxygen consumption, and calcium retention capacity. Mitochondrial function decreased significantly after ischemia and showed mild improvement with reperfusion. P188 did not improve mitochondrial function in the ex-vivo heart, and neither further P188 nor PEG induced direct mitochondrial protection after IR injury in this model. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc261dd9f89c8994b1a82287573613fbTest http://europepmc.org/articles/PMC8124240Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bc261dd9f89c8994b1a82287573613fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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