Enhancing the function of CD34(+) cells by targeting plasminogen activator inhibitor-1
| العنوان: | Enhancing the function of CD34(+) cells by targeting plasminogen activator inhibitor-1 |
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| المؤلفون: | Carl J. Pepine, Sergio Caballero, Elena Nikonova, Maria B. Grant, Paul J. Higgins, Sugata Hazra, Stephen H. Bartelmez, Valerie Stepps, David J. Stone, Catherine Thut, Michael E. Boulton, Ashay D Bhatwadekar, Eva M. Finney |
| المصدر: | PLoS ONE, Vol 8, Iss 11, p e79067 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cell, CD34, lcsh:Medicine, Antigens, CD34, 030204 cardiovascular system & hematology, Cohort Studies, Mice, Phosphatidylinositol 3-Kinases, chemistry.chemical_compound, 0302 clinical medicine, Cell Movement, lcsh:Science, Cyclic GMP, Cells, Cultured, Oligonucleotide Array Sequence Analysis, 0303 health sciences, Multidisciplinary, Reverse Transcriptase Polymerase Chain Reaction, Middle Aged, 3. Good health, medicine.anatomical_structure, Reperfusion Injury, Plasminogen activator inhibitor-1, RNA Interference, Research Article, Adult, medicine.medical_specialty, Diabetic angiopathy, Transforming Growth Factor beta1, 03 medical and health sciences, Internal medicine, Plasminogen Activator Inhibitor 1, Diabetes Mellitus, medicine, Animals, Humans, Progenitor cell, Cell Proliferation, 030304 developmental biology, Cell growth, lcsh:R, Retinal Vessels, medicine.disease, Molecular biology, Endocrinology, chemistry, Leukocytes, Mononuclear, lcsh:Q, Transcriptome, Reperfusion injury, Plasminogen activator, Diabetic Angiopathies |
| الوصف: | Previously, we showed that transient inhibition of TGF- β1 resulted in correction of key aspects of diabetes-induced CD34(+) cell dysfunction. In this report, we examine the effect of transient inhibition of plasminogen activator inhibitor-1 (PAI-1), a major gene target of TGF-β1 activation. Using gene array studies, we examined CD34(+) cells isolated from a cohort of longstanding diabetic individuals, free of microvascular complications despite suboptimal glycemic control, and found that the cells exhibited reduced transcripts of both TGF-β1 and PAI-1 compared to age, sex, and degree of glycemic control-matched diabetic individuals with microvascular complications. CD34(+) cells from diabetic subjects with microvascular complications consistently exhibited higher PAI-1 mRNA than age-matched non-diabetic controls. TGF- β1 phosphorodiamidate morpholino oligo (PMO) reduced PAI-1 mRNA in diabetic (p |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbd0dbde606b9513989e8379b7f118d8Test http://europepmc.org/articles/PMC3815099?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bbd0dbde606b9513989e8379b7f118d8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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