Lipidome Analysis in Brain and Peripheral Plasma Following Milk Fat Globule Membrane Supplementation in Rodents
| العنوان: | Lipidome Analysis in Brain and Peripheral Plasma Following Milk Fat Globule Membrane Supplementation in Rodents |
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| المؤلفون: | Rosalind Davies, Janna A. van Diepen, Lauren R. Brink, Sabina Bijlsma, Karen‐Anne McVey Neufeld, John F. Cryan, Siobhain M. O'Mahony, Ivana Bobeldijk, Gabriele Gross |
| المصدر: | Molecular Nutrition & Food Research (Formerly Nahrung/Food), 66 Molecular Nutrition & Food Research (Formerly Nahrung/Food), 66, 22 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Brain, Metabolic Disorders Radboud Institute for Molecular Life Sciences [Radboudumc 6], Lipid Droplets, Blood plasma, Rodents, Rats, Sphingomyelins, Rats, Sprague-Dawley, Mice, Milk fat globule membrane, Dietary Supplements, Lipidomics, Animals, Glycolipids, Phospholipids, Glycoproteins, Food Science, Biotechnology |
| الوصف: | Scope: Milk fat globule membrane (MFGM) is an essential component of milk. Bovine MFGM (bMFGM) has been shown to support cognitive development and increase relative concentrations of serum phospholipids. This study investigated bioavailability of bMFGM components after oral administration in two preclinical models to explore whether dietary bMFGM induced parallel changes to plasma and brain lipidomes. Methods and results: Transgenic APOE*3.Leiden mice (n = 18/group) and Sprague-Dawley rats (n = 12/group) were fed bMFGM-enriched (MFGM+) or Control diet, after which phospholipid profiles were determined in peripheral plasma, hippocampus and prefrontal cortex tissue by targeted mass spectrometry. Multivariate analysis of lipidomic profiles demonstrated a clear separation between MFGM+ and Control plasma samples across rodents. In plasma, sphingomyelins contributed the most to the separation of lipid patterns among both models, where three sphingomyelins (d18:1/14:0, d18:1/23:0, d18:1/23:1[9Z]) were significantly and consistently higher in the circulation of MFGM+ versus Control groups. A similar trend was observed in rat prefrontal cortex, although no significant separation of the brain lipidome was demonstrated. Conclusion: bMFGM-enriched diet alters plasma phospholipid composition in rodents, predominantly increasing sphingomyelin levels in the systemic circulation with some similar, but non-significant, trends in central regions of the brain. These changes may contribute to the beneficial effects of dietary bMFGM on neurodevelopment during early life. |
| وصف الملف: | application/pdf |
| تدمد: | 1613-4133 1613-4125 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb5cbab6372446fd741b4ef3689a810dTest https://doi.org/10.1002/mnfr.202200177Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bb5cbab6372446fd741b4ef3689a810d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16134133 16134125 |
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