Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients
| العنوان: | Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients |
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| المؤلفون: | Flavia Franconi, Zaleida Napoli, Loria Bianchi, Giuseppe Seghieri, Angelo Zinellu, Ilaria Campesi, Alessandra De Bellis, Stefano Occhioni, Ciriaco Carru, Roberto Anichini |
| المصدر: | Journal of Diabetes Research, Vol 2016 (2016) Journal of Diabetes Research |
| بيانات النشر: | Hindawi Limited, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, medicine.medical_specialty, Taurine, Homocysteine, Article Subject, Endocrinology, Diabetes and Metabolism, medicine.disease_cause, lcsh:Diseases of the endocrine glands. Clinical endocrinology, 03 medical and health sciences, chemistry.chemical_compound, Endocrinology, Internal medicine, Diabetes mellitus, medicine, Humans, RNA, Messenger, Endothelial dysfunction, Glycated Hemoglobin, Type 1 diabetes, Diabetic Retinopathy, Membrane Glycoproteins, lcsh:RC648-665, business.industry, Membrane Transport Proteins, Diabetic retinopathy, Middle Aged, medicine.disease, Up-Regulation, Oxidative Stress, 030104 developmental biology, Diabetes Mellitus, Type 1, chemistry, Case-Control Studies, Leukocytes, Mononuclear, Female, Endothelium, Vascular, business, Oxidative stress, Biomarkers, Retinopathy, Research Article |
| الوصف: | Background. Taurine transporter gene expression (RNA-TauT) has a role in retinal cell function and is modulatedin vitroandin vivoby hyperglycemia and/or oxidative stress. This study was aimed at testing whether RNA-TauT gene expression is modified in blood mononuclear peripheral cells (MPCs) of type 1 diabetic patients, is related to plasma markers of oxidative stress or endothelial dysfunction, or, finally, is related to presence of retinopathy.Methods. RNA-TauT was measured in MPCs by real-time PCR-analysis in 35 type 1 diabetic patients and in 33 age- and sex-matched controls, additionally measuring plasma and cell taurine and markers of oxidative stress and endothelial dysfunction.Results. RNA-TauT, expressed as2-ΔΔCt, was significantly higher in MPCs of type 1 diabetic patients than in controls [median (interquartile range): 1.32(0.31) versus 1.00(0.15);P=0.01]. In diabetic patients RNA-TauT was related to HbA1c (r=0.42;P=0.01) and inversely to plasma homocysteine (r=-0.39;P=0.02) being additionally significantly higher in MPCs of patients without retinopathy [(n=22); 1.36(0.34)] compared to those with retinopathy [(n=13); 1.16(0.20)], independently from HbA1c or diabetes duration.Conclusions. RNA-TauT gene expression is significantly upregulated in MPCs of type 1 diabetes patients and is related to HbA1c levels and inversely to plasma homocysteine. Finally, in diabetes patients, RNA-TauT upregulation seems to be blunted in patients with retinopathy independently of their metabolic control or longer diabetes duration. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-6753 2314-6745 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9a387e31daf84161a3c2a68340578abTest https://doaj.org/article/1b0ef184ed8548e8b92aa8a35bce0469Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b9a387e31daf84161a3c2a68340578ab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146753 23146745 |
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