Hydrogen gas alleviates oxygen toxicity by reducing hydroxyl radical levels in PC12 cells
| العنوان: | Hydrogen gas alleviates oxygen toxicity by reducing hydroxyl radical levels in PC12 cells |
|---|---|
| المؤلفون: | Junchao Yu, Lianbi Xue, Ruiyun Zhang, Yaling Liu, Qiuhong Yu |
| المصدر: | PLoS ONE PLoS ONE, Vol 12, Iss 3, p e0173645 (2017) |
| بيانات النشر: | Public Library of Science, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Antioxidant, medicine.medical_treatment, lcsh:Medicine, Pharmacology, medicine.disease_cause, Oxygen, Biochemistry, PC12 Cells, Antioxidants, Lipid peroxidation, chemistry.chemical_compound, Oxidative Damage, 0302 clinical medicine, Medicine and Health Sciences, lcsh:Science, Oxygen toxicity, Energy-Producing Organelles, chemistry.chemical_classification, Fluids, Antioxidant Therapy, Multidisciplinary, Pharmaceutics, Physics, Chemical Reactions, Mitochondria, Chemistry, 030220 oncology & carcinogenesis, Physical Sciences, Gases, Cellular Structures and Organelles, Research Article, Chemical Elements, States of Matter, Cell Survival, chemistry.chemical_element, Bioenergetics, 03 medical and health sciences, Drug Therapy, Oxidation, medicine, Animals, Reactive oxygen species, L-Lactate Dehydrogenase, Hydroxyl Radical, lcsh:R, Biology and Life Sciences, DNA oxidation, Cell Biology, medicine.disease, Rats, chemistry, Hydroxyl radical, lcsh:Q, Lipid Peroxidation, Reactive Oxygen Species, 030217 neurology & neurosurgery, Oxidative stress, Hydrogen |
| الوصف: | Hyperbaric oxygen (HBO) therapy through breathing oxygen at the pressure of above 1 atmosphere absolute (ATA) is useful for varieties of clinical conditions, especially hypoxic-ischemic diseases. Because of generation of reactive oxygen species (ROS), breathing oxygen gas at high pressures can cause oxygen toxicity in the central nervous system, leading to multiple neurological dysfunction, which limits the use of HBO therapy. Studies have shown that Hydrogen gas (H2) can diminish oxidative stress and effectively reduce active ROS associated with diseases. However, the effect of H2 on ROS generated from HBO therapy remains unclear. In this study, we investigated the effect of H2 on ROS during HBO therapy using PC12 cells. PC12 cells cultured in medium were exposed to oxygen gas or mixed oxygen gas and H2 at 1 ATA or 5 ATA. Cells viability and oxidation products and ROS were determined. The data showed that H2 promoted the cell viability and inhibited the damage in the cell and mitochondria membrane, reduced the levels of lipid peroxidation and DNA oxidation, and selectively decreased the levels of •OH but not disturbing the levels of O2•-, H2O2, or NO• in PC12 cells during HBO therapy. These results indicated that H2 effectively reduced •OH, protected cells against oxygen toxicity resulting from HBO therapy, and had no effect on other ROS. Our data supported that H2 could be potentially used as an antioxidant during HBO therapy. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b92604f3a95fe60af16767c85a5055b2Test http://europepmc.org/articles/PMC5375132Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b92604f3a95fe60af16767c85a5055b2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|