Extensive conformational sampling in a ternary electron transfer complex
| العنوان: | Extensive conformational sampling in a ternary electron transfer complex |
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| المؤلفون: | Jaswir Basran, Nigel S. Scrutton, David Leys, Michael J. Sutcliffe, François Talfournier |
| المصدر: | Nature Structural & Molecular Biology. 10:219-225 |
| بيانات النشر: | Springer Science and Business Media LLC, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Models, Molecular, Electron density, Electron-Transferring Flavoproteins, Macromolecular Substances, Protein Conformation, Molecular Sequence Data, Crystallography, X-Ray, Electron-transferring flavoprotein, Redox, Molecular dynamics, Electron transfer, Structural Biology, Amino Acid Sequence, Molecular Biology, Flavoproteins, Sequence Homology, Amino Acid, biology, Chemistry, Oxidoreductases, N-Demethylating, Electron transport chain, Protein Structure, Tertiary, Kinetics, Crystallography, Methylophilus methylotrophus, Flavin-Adenine Dinucleotide, biology.protein, Trimethylamine dehydrogenase, Ternary operation |
| الوصف: | Here we report the crystal structures of a ternary electron transfer complex showing extensive motion at the protein interface. This physiological complex comprises the iron-sulfur flavoprotein trimethylamine dehydrogenase and electron transferring flavoprotein (ETF) from Methylophilus methylotrophus. In addition, we report the crystal structure of free ETF. In the complex, electron density for the FAD domain of ETF is absent, indicating high mobility. Positions for the FAD domain are revealed by molecular dynamics simulation, consistent with crystal structures and kinetic data. A dual interaction of ETF with trimethylamine dehydrogenase provides for dynamical motion at the protein interface: one site acts as an anchor, thereby allowing the other site to sample a large range of interactions, some compatible with rapid electron transfer. This study establishes the role of conformational sampling in multi-domain redox systems, providing insight into electron transfer between ETFs and structurally distinct redox partners. |
| تدمد: | 1545-9985 1545-9993 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b77c4ea08b4855bf69a6c1032fe24c04Test https://doi.org/10.1038/nsb894Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b77c4ea08b4855bf69a6c1032fe24c04 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15459985 15459993 |
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