Molecular Detection of Spirometra decipiens in the United States
| العنوان: | Molecular Detection of Spirometra decipiens in the United States |
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| المؤلفون: | Keeseon S. Eom, Dongmin Lee, Woon Mok Sohn, Hansol Park, Hyeong-Kyu Jeon, Seongjun Choe |
| المصدر: | The Korean Journal of Parasitology |
| بيانات النشر: | Korean Society for Parasitology, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Sequence Homology, Zoology, nad3, Electron Transport Complex IV, 03 medical and health sciences, Monophyly, Phylogenetics, Plerocercoid, Animals, Cluster Analysis, Spirometra, USA, Phylogeny, Diphyllobothrium, Microscopy, Pseudophyllidea, biology, Phylogenetic tree, sparganum, Animal Structures, NADH Dehydrogenase, Sequence Analysis, DNA, Anatomy, 030108 mycology & parasitology, Spirometra decipiens, biology.organism_classification, cox1, United States, Maximum parsimony, molecular detection, 030104 developmental biology, Infectious Diseases, Original Article, Parasitology |
| الوصف: | The genus Spirometra belongs to the family Diphyllobothriidae and order Pseudophyllidea, and includes intestinal parasites of cats and dogs. In this study, a plerocercoid labeled as Spirometra mansonoides from the USA was examined for species identification and phylogenetic analysis using 2 complete mitochondrial genes, cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit 3 (nad3). The cox1 sequences (1,566 bp) of the plerocercoid specimen (USA) showed 99.2% similarity to the reference sequences of the plerocercoid of Korean Spirometra decipiens (GenBank no. KJ599679), and 99.1% similarity in regard to nad3 (346 bp). Phylogenetic tree topologies generated using 4 analytical methods were identical and showed high confidence levels with bootstrap values of 1.00, 100%, 100%, and 100% for Bayesian inference (BI), maximum-likelihood (ML), neighbor-joining (NJ), and maximum parsimony (MP) methods, respectively. Representatives of Diphyllobothrium and Spirometra species formed a monophyletic group, and the sister-genera status between these species was well supported. Trapezoic proglottids in the posterior 1/5 region of an adult worm obtained from an experimentally infected cat were morphologically examined. The outer uterine loop of the uterus coiling characteristically consisted of 2 complete turns. The results clearly indicated that the examined Spirometra specimen from the USA matched to S. decipiens very well, and indicated possible presence of the life cycle of this species in this region. |
| تدمد: | 1738-0006 0023-4001 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b75d45d777f306ed08023e1345e9ed96Test https://doi.org/10.3347/kjp.2016.54.4.503Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b75d45d777f306ed08023e1345e9ed96 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17380006 00234001 |
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