Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands
| العنوان: | Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands |
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| المؤلفون: | Luen Hwu, Cheau-Ling Ho, Min-Tzu Ku, Ren Shyan Liu, Wen-Yi Chang, Yi-An Chen, Sain-Jhih Chiu, Cheng-Hsiu Lu |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Epithelial-Mesenchymal Transition, Science, Cell, Mice, Nude, Ligands, Article, Metastasis, Mice, Cancer stem cell, Cell Line, Tumor, Spheroids, Cellular, Biomarkers, Tumor, medicine, Animals, Humans, Vimentin, Dimethyl Sulfoxide, Cell Proliferation, Cancer, Mice, Inbred BALB C, Reporter gene, Multidisciplinary, Chemistry, Transfection, Cell sorting, medicine.disease, Xenograft Model Antitumor Assays, Tongue Neoplasms, Tumor Burden, Squamous carcinoma, medicine.anatomical_structure, embryonic structures, Carcinoma, Squamous Cell, Neoplastic Stem Cells, Quinazolines, Cancer research, Medicine, Peptides, Nucleolin, Biomarkers |
| الوصف: | The occurrence of epithelial-mesenchymal transition (EMT) within tumors, which enables invasion and metastasis, is linked to cancer stem cells (CSCs) with drug and radiation resistance. We used two specific peptides, F7 and SP peptides, to detect EMT derived cells or CSCs. Human tongue squamous carcinoma cell line-SAS transfected with reporter genes was generated and followed by spheroid culture. A small molecule inhibitor-Unc0642 and low-dose ionizing radiation (IR) were used for induction of EMT. Confocal microscopic imaging and fluorescence-activated cell sorting analysis were performed to evaluate the binding ability and specificity of peptides. A SAS xenograft mouse model with EMT induction was established for assessing the binding affinity of peptides. The results showed that F7 and SP peptides not only specifically penetrated into cytoplasm of SAS cells but also bound to EMT derived cells and CSCs with high nucleolin and vimentin expression. In addition, the expression of CSC marker and the binding of peptides were increased in tumors isolated from Unc0642/IR-treated groups. Our study demonstrates the potential of these peptides for detecting EMT derived cells or CSCs and might provide an alternative isolation method for these subpopulations within the tumor in the future. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b737df2cbff87f45e3d3a766a570ffccTest https://doaj.org/article/7223393fcab74b7685835304f7d72b7cTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b737df2cbff87f45e3d3a766a570ffcc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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