Osteochondral regeneration using a novel aragonite-hyaluronate bi-phasic scaffold in a goat model
| العنوان: | Osteochondral regeneration using a novel aragonite-hyaluronate bi-phasic scaffold in a goat model |
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| المؤلفون: | Andrew Levy, Dror Robinson, Jonathan Shani, Nir Altschuler, Elizaveta Kon, Giuseppe Filardo, Kenneth Zaslav, John A. Eisman |
| المساهمون: | E. Kon, G. Filardo, D. Robinson, J. A. Eisman, A. Levy, K. Zaslav, J. Shani, N. Altschuler |
| المصدر: | Knee Surgery, Sports Traumatology, Arthroscopy. 22:1452-1464 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cartilage, Articular, Scaffold, osteochondral regeneration, Biocompatible Materials, Bone healing, Bone and Bones, Calcium Carbonate, chemistry.chemical_compound, Hyaluronic acid, Animals, Regeneration, Medicine, Orthopedics and Sports Medicine, Hyaluronic Acid, Bone regeneration, Wound Healing, Tissue Scaffolds, business.industry, Hyaline cartilage, Goats, Cartilage, Regeneration (biology), Prostheses and Implants, Anatomy, Anthozoa, medicine.anatomical_structure, chemistry, Models, Animal, Surgery, Implant, business |
| الوصف: | PURPOSE: The objective of this study was to examine whether different mechanical modifications and/or impregnation of hyaluronic acid (HA) might enhance aragonite-based scaffold properties for the regeneration of cartilage and bone in an animal model. METHODS: Bi-phasic osteochondral scaffolds were prepared using coralline aragonite with different modifications, including 1- to 2-mm-deep drilled channels in the cartilage phase (Group 1, n = 7) or in the bone phase (Group 2, n = 8), and compared with unmodified coral cylinders (Group 3, n = 8) as well as empty control defects (Group 4, n = 4). In each group, four of the implants were impregnated with HA to the cartilage phase. Osteochondral defects (6 mm diameter, 8 mm depth) were made in medial and lateral femoral condyles of 14 goats, and the scaffolds were implanted according to a randomization chart. After 6 months, cartilage and bone regeneration were evaluated macroscopically and histologically by an external laboratory. RESULTS: Group 1 implants were replaced by newly formed hyaline cartilage and subchondral bone (combined histological evaluation according to the ICRS II-2010 and O'Driscoll et al. 34 ± 4 n = 7). In this group, the cartilaginous repair tissue showed a smooth contour and was well integrated into the adjacent native cartilage, with morphological evidence of hyaline cartilage as confirmed by the marked presence of proteoglycans, a marked grade of collagen type II and the absence of collagen type I. The average scores in other groups were significantly lower (Group 2 (n = 8) 28.8 ± 11, Group 3 (n = 8) 23 ± 9 and Group 4 (empty control, n = 4) 19.7 ± 15). CONCLUSIONS: The implants with the mechanical modification and HA impregnation in the cartilage phase outperformed all other types of implant. Although native coral is an excellent material for bone repair, as a stand-alone material implant, it does not regenerate hyaline cartilage. Mechanical modification with drilled channels and impregnation of HA within the coral pores enhanced the scaffold's cartilage regenerative potential. The modified implant shows young hyaline cartilage regeneration. This implant might be useful for the treatment of both chondral and osteochondral defects in humans. |
| وصف الملف: | STAMPA |
| تدمد: | 1433-7347 0942-2056 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6f482b04621898312bb7b49cbc3cc50Test https://doi.org/10.1007/s00167-013-2467-2Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b6f482b04621898312bb7b49cbc3cc50 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14337347 09422056 |
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