Sonic Hedgehog Has a Dual Effect on the Growth of Retinal Ganglion Axons Depending on Its Concentration
| العنوان: | Sonic Hedgehog Has a Dual Effect on the Growth of Retinal Ganglion Axons Depending on Its Concentration |
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| المؤلفون: | Jinhua Zhang, Zheng-Zheng Bao, Adrianne L. Kolpak |
| المصدر: | The Journal of Neuroscience. 25:3432-3441 |
| بيانات النشر: | Society for Neuroscience, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Retinal Ganglion Cells, Time Factors, animal structures, genetic structures, Green Fluorescent Proteins, Growth Cones, Optic Disk, Fluorescent Antibody Technique, Chick Embryo, Retinal ganglion, Retina, Article, Organ Culture Techniques, medicine, Animals, Drug Interactions, Hedgehog Proteins, RNA, Messenger, Sonic hedgehog, Ganglion cell layer, Cells, Cultured, In Situ Hybridization, Regulation of gene expression, Dose-Response Relationship, Drug, biology, General Neuroscience, Age Factors, Veratrum Alkaloids, Gene Expression Regulation, Developmental, Neural Inhibition, Axons, Coculture Techniques, Recombinant Proteins, eye diseases, Cell biology, medicine.anatomical_structure, Retinal ganglion cell, embryonic structures, Trans-Activators, biology.protein, Axon guidance, sense organs, Neuroscience, Optic disc |
| الوصف: | The stereotypical projection of retinal ganglion cell (RGC) axons to the optic disc has served as a good model system for studying axon guidance. By bothin vitroandin vivoexperiments, we show that a secreted molecule, Sonic hedgehog (Shh), may play a critical role in the process. It is expressed in a dynamic pattern in the ganglion cell layer with a relatively higher expression in the center of the retina. Through gel culture and stripe assays, we show that Shh has a dual effect on RGC axonal growth, acting as a positive factor at low concentrations and a negative factor at high concentrations. Results from time-lapse video microscopic and stripe assay experiments further suggest that the effects of Shh on axons are not likely attributable to indirect transcriptional regulation by Shh. Overexpression of Shh protein or inhibition of Shh function inside the retina resulted in a complete loss of centrally directed projection of RGC axons, suggesting that precise regulation of Shh level inside the retina is critical for the projection of RGC axons to the optic disc. |
| تدمد: | 1529-2401 0270-6474 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6a5f3f7af0e8a5a072b37d678f0261bTest https://doi.org/10.1523/jneurosci.4938-04.2005Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b6a5f3f7af0e8a5a072b37d678f0261b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292401 02706474 |
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