Chitinase effects on immune cell response in neuromyelitis optica and multiple sclerosis
| العنوان: | Chitinase effects on immune cell response in neuromyelitis optica and multiple sclerosis |
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| المؤلفون: | Marcela Fiol, Jorge Correale |
| المصدر: | Multiple Sclerosis Journal. 17:521-531 |
| بيانات النشر: | SAGE Publications, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Adult, Male, Enzyme-Linked Immunospot Assay, Cellular immunity, Pathology, medicine.medical_specialty, Argentina, Arthritis, Enzyme-Linked Immunosorbent Assay, Inflammatory bowel disease, CHI3L1, Multiple Sclerosis, Relapsing-Remitting, Immune system, Adipokines, Lectins, medicine, Humans, Chitinase-3-Like Protein 1, Cells, Cultured, Glycoproteins, Neuromyelitis optica, business.industry, Multiple sclerosis, ELISPOT, Chitinases, Neuromyelitis Optica, Transendothelial and Transepithelial Migration, Endothelial Cells, Multiple Sclerosis, Chronic Progressive, medicine.disease, Coculture Techniques, Up-Regulation, Myelin-Associated Glycoprotein, Hexosaminidases, Neurology, Blood-Brain Barrier, Case-Control Studies, Immunology, Leukocytes, Mononuclear, Cytokines, Female, Myelin-Oligodendrocyte Glycoprotein, Neurology (clinical), Chemokines, business, Biomarkers, Myelin Proteins |
| الوصف: | Background: Recent studies conducted in arthritis, asthma, and inflammatory bowel disease suggest that chitinases are important in inflammatory processes and tissue remodeling. Objective: To investigate the role of chitinases in multiple sclerosis (MS) and neuromyelitis optica (NMO). Methods: Levels of chitotriosidase, acid mammalian chitinase (AMCase), and chitinase 3-like-1 (CHI3L1) were measured using ELISA, in cerebrospinal fluid (CSF) and in serum from 24 patients with relapsing remitting (RR) MS, 24 patients with secondary progressive (SP) MS, 12 patients with NMO, 24 patients with other inflammatory neurological diseases (OIND), and 24 healthy controls (HCs). The number of anti-MOG cytokine-secreting cells was studied using ELISPOT. Eotaxins, MCP-1, RANTES, and IL-8 were assessed using ELISA. Cell transmigration was determined using an in vitro blood–brain barrier (BBB) model, in the presence and absence of chitinases. Results: CSF chitinase levels were significantly increased in patients with RRMS and NMO compared with HCs and patients with SPMS and OIND. In contrast, no significant differences were detected in serum chitinase levels between groups. Chitinase CSF levels showed correlation with anti-MOG IL-13-producing cells, and eotaxin levels. In vitro experiments showed macrophage chitinase secretion was significantly increased by IL-13, but not by IL-5, IL-6, IL-12, or IFN-γ. Moreover, chitinases enhanced IL-8, RANTES, MCP-1, and eotaxin production, increasing migratory capacity in eosinophils, T cells, and macrophages across an in vitro BBB model. Conclusions: Chitinases increased in the CSF from patients with NMO in response to IL-13. These enhanced levels could contribute to central nervous system inflammation by increasing immune cell migration across the BBB. |
| تدمد: | 1477-0970 1352-4585 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b65f48966a7d976f6c5218fce224544eTest https://doi.org/10.1177/1352458510392619Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b65f48966a7d976f6c5218fce224544e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14770970 13524585 |
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