Live Imaging Reveals Listeria Hijacking of E-Cadherin Recycling as It Crosses the Intestinal Barrier
| العنوان: | Live Imaging Reveals Listeria Hijacking of E-Cadherin Recycling as It Crosses the Intestinal Barrier |
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| المؤلفون: | Cindy Fevre, Marc Lecuit, Minhee Kim, Olivier Disson, Morgane Lavina |
| المساهمون: | Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Minhee Kim received a long-term postdoctoral fellowship from the European Molecular Biology Organization (EMBO), ALTF 1192-2017. Institut Pasteur, Inserm, LabEx IBEID, the European Research Council (ERC) Invadis and ANR Organolist provided financial support., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-15-CE15-0007,OrganoList,Interactions entre Listeria monocytogenes et la barrière intestinale(2015), European Project: 681829,ERC-CoG-2015,INVADIS(2016), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Current Biology-CB Current Biology-CB, 2021, 31 (5), pp.1037-1047.e4. ⟨10.1016/j.cub.2020.11.041⟩ Current Biology-CB, Elsevier, 2021, 31 (5), pp.1037-1047.e4. ⟨10.1016/j.cub.2020.11.041⟩ Current Biology |
| بيانات النشر: | HAL CCSD, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, E-cadherin recycling, Listeria, [SDV]Life Sciences [q-bio], Vacuole, Biology, Endocytosis, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Bacterial Proteins, Listeria monocytogenes, Live cell imaging, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, medicine, Humans, Listeriosis, Internalin, intestine, organoids, Goblet cell, goblet cell, live imaging, Cadherins, Intestinal epithelium, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Transcytosis, Rab11, epithelial translocation, General Agricultural and Biological Sciences, 030217 neurology & neurosurgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience; Listeria monocytogenes is a foodborne bacterial pathogen that causes human listeriosis, a severe systemic infection [1]. Its translocation across the intestinal epithelium is mediated by the interaction of internalin (InlA), a Listeria surface protein, with its host species-specific receptor E-cadherin (Ecad) [2-5]. It occurs through goblet cells, on which Ecad is luminally accessible [6], via an unknown mechanism. In the absence of cell lines recapitulating this phenotype in vitro, we developed an ex vivo experimental system, based on the intraluminal microinjection of Listeria in untreated, pharmacologically-treated and genetically modified intestinal organoids. Using both live light sheet microscopy and confocal imaging, we show that Listeria translocates through goblet cells within a membrane vacuole in an InlA-and microtubule-dependent manner. As Ecad undergoes constant apical-basal recycling [7, 8], we hypothesized that Lm may transit through goblet cells by hijacking Ecad recycling pathway. Indeed, Listeria is stuck at goblet cell apex when Ecad endocytosis is blocked, and remains trapped intracellularly at the basolateral pole of goblet cells when Rab11-dependent Ecad recycling is compromised. Together, these results show that Listeria, upon docking onto its luminally accessible receptor Ecad, hijacks its recycling pathway to be transferred by transcytosis across goblet cells. Live imaging of host-pathogen interactions in organoids is a promising approach to dissect their underlying cell and molecular biology. |
| اللغة: | English |
| تدمد: | 0960-9822 1879-0445 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1e2aa6bafca71db8f65c67edc4f9803Test https://hal-pasteur.archives-ouvertes.fr/pasteur-03271351/documentTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b1e2aa6bafca71db8f65c67edc4f9803 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09609822 18790445 |
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