Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation
| العنوان: | Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation |
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| المؤلفون: | Martin J. Hoogduijn, Carla C. Baan, L.J.W. van der Laan, Wenda Verschoor, Robert C. Minnee, Jeroen G. H. P. Verhoeven, Dennis A. Hesselink, M. W. F. van den Hoogen, H. Tejeda-Mora, Karin Boer |
| المساهمون: | Internal Medicine, Surgery |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports, 11(1). Nature Publishing Group Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Graft Rejection, Male, 0301 basic medicine, Nephrology, medicine.medical_specialty, Pathology, Time Factors, Science, Human leukocyte antigen, 030230 surgery, Article, 03 medical and health sciences, Medical research, 0302 clinical medicine, Internal medicine, Biopsy, medicine, Humans, Hepatitis A Virus Cellular Receptor 1, Kidney transplantation, Kidney, Multidisciplinary, medicine.diagnostic_test, business.industry, Endothelial Cells, Middle Aged, Allografts, Flow Cytometry, medicine.disease, Kidney Transplantation, Phenotype, Transplantation, 030104 developmental biology, medicine.anatomical_structure, surgical procedures, operative, cardiovascular system, Biomarker (medicine), Medicine, Female, business, Biomarkers |
| الوصف: | The diagnosis of kidney allograft rejection is based on late histological and clinical markers. Early, specific and minimally-invasive biomarkers may improve rejection diagnosis. Endothelial cells (EC) are one of the earliest targets in kidney transplant rejection. We investigated whether circulating EC (cEC) could serve as an earlier and less invasive biomarker for allograft rejection. Blood was collected from a cohort of 51 kidney transplant recipients before and at multiple timepoints after transplantation, including during a for cause biopsy. The number and phenotype of EC was assessed by flow-cytometric analysis. Unbiased selection of EC was done using principal component (PCA) analysis. Paired analysis revealed a transient cEC increase of 2.1-fold on the third day post-transplant, recovering to preoperative levels at seventh day post-transplant and onwards. Analysis of HLA subtype demonstrated that cEC mainly originate from the recipient. cEC levels were not associated with allograft rejection, allograft function or other allograft pathologies. However, cEC in patients with allograft rejection and increased levels of cEC showed elevated levels of KIM-1 (kidney injury marker-1). These findings indicate that cEC numbers and phenotype are affected after kidney transplantation but may not improve rejection diagnosis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0fa604b49527466012368fd4f309e4bTest https://doi.org/10.1038/s41598-021-88411-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b0fa604b49527466012368fd4f309e4b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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