A combined approach for single-cell mRNA and intracellular protein expression analysis
| العنوان: | A combined approach for single-cell mRNA and intracellular protein expression analysis |
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| المؤلفون: | Niklas Dahl, Marcel Tarbier, Sathishkumar Baskaran, Marcus Danielsson, Styliani Panagiotou, Caroline J. Gallant, Johan Reimegård, Marc R. Friedländer, Jens Schuster |
| المصدر: | Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021) Communications Biology |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Proteomics, Cell biology, Transcription, Genetic, QH301-705.5, Sequence analysis, Cell- och molekylärbiologi, Cell, Medicine (miscellaneous), Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Transcription (biology), medicine, Humans, RNA, Messenger, Biology (General), Transcriptomics, Transcription factor, 030304 developmental biology, 0303 health sciences, Messenger RNA, Sequence Analysis, RNA, Intracellular protein, Chemistry, Gene Expression Profiling, Proteins, RNA, Protein subcellular localization prediction, medicine.anatomical_structure, Single-Cell Analysis, General Agricultural and Biological Sciences, Cell and Molecular Biology, 030217 neurology & neurosurgery |
| الوصف: | Combined measurements of mRNA and protein expression in single cells enable in-depth analysis of cellular states. We present SPARC, an approach that combines single-cell RNA-sequencing with proximity extension essays to simultaneously measure global mRNA and 89 intracellular proteins in individual cells. We show that mRNA expression fails to accurately reflect protein abundance at the time of measurement, although the direction of changes is in agreement during neuronal differentiation. Moreover, protein levels of transcription factors better predict their downstream effects than do their corresponding transcripts. Finally, we highlight that protein expression variation is overall lower than mRNA variation, but relative protein variation does not reflect the mRNA level. Our results demonstrate that mRNA and protein measurements in single cells provide different and complementary information regarding cell states. SPARC presents a state-of-the-art co-profiling method that overcomes current limitations in throughput and protein localization, including removing the need for cell fixation. Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor predictors of protein abundances and activity in individual cells, indicating that the measurements are complementary. |
| وصف الملف: | application/pdf |
| تدمد: | 2399-3642 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afdab16ab54c1c12205dbd93d59f0587Test https://doi.org/10.1038/s42003-021-02142-wTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....afdab16ab54c1c12205dbd93d59f0587 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23993642 |
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