A Structurally Simple Vaccine Candidate Reduces Progression and Dissemination of Triple-Negative Breast Cancer
| العنوان: | A Structurally Simple Vaccine Candidate Reduces Progression and Dissemination of Triple-Negative Breast Cancer |
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| المؤلفون: | Amedei, Amedeo, Asadzadeh, Fatemeh, Papi, Francesco, Vannucchi, Maria Giuliana, Ferrucci, Veronica, Bermejo, Iris A., Fragai, Marco, De Almeida, Carolina Vieira, Cerofolini, Linda, Giuntini, Stefano, Bombaci, Mauro, Pesce, Elisa, Niccolai, Elena, Natali, Francesca, Guarini, Eleonora, Gabel, Frank, Traini, Chiara, Catarinicchia, Stefano, Ricci, Federica, Orzalesi, Lorenzo, Berti, Francesco, Corzana, Francisco, Zollo, Massimo, Grifantini, Renata, Nativi, Cristina, 0000-0002-2143-046X, 0000-0001-8864-4852, 0000-0002-0795-9594, 0000-0002-1631-4624, 0000-0001-5597-8127, 0000-0002-6312-3230 |
| المساهمون: | Amedei, A., Asadzadeh, F., Papi, F., Vannucchi, M. G., Ferrucci, V., Bermejo, I. A., Fragai, M., De Almeida, C. V., Cerofolini, L., Giuntini, S., Bombaci, M., Pesce, E., Niccolai, E., Natali, F., Guarini, E., Gabel, F., Traini, C., Catarinicchia, S., Ricci, F., Orzalesi, L., Berti, F., Corzana, F., Zollo, M., Grifantini, R., Nativi, C. |
| المصدر: | iScience (Cambridge) Online 23 (2020). doi:10.1016/j.isci.2020.101250 info:cnr-pdr/source/autori:Amedei, Amedeo; Asadzadeh, Fatemeh; Papi, Francesco; Vannucchi, Maria Giuliana; Ferrucci, Veronica; Bermejo, Iris A.; Fragai, Marco; De Almeida, Carolina Vieira; Cerofolini, Linda; Giuntini, Stefano; Bombaci, Mauro; Pesce, Elisa; Niccolai, Elena; Natali, Francesca; Guarini, Eleonora; Gabel, Frank; Traini, Chiara; Catarinicchia, Stefano; Ricci, Federica; Orzalesi, Lorenzo; Berti, Francesco; Corzana, Francisco; Zollo, Massimo; Grifantini, Renata; Nativi, Cristina/titolo:A Structurally Simple Vaccine Candidate Reduces Progression and Dissemination of Triple-Negative Breast Cancer/doi:10.1016%2Fj.isci.2020.101250/rivista:iScience (Cambridge) Online/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:23 iScience 'iScience ', vol: 23, pages: 101250-1-101250-53 (2020) iScience, Vol 23, Iss 6, Pp 101250-(2020) RIUR. Repositorio Institucional de la Universidad de La Rioja instname |
| بيانات النشر: | Cell Press Elsevier Inc., [Cambridge MA], Paesi Bassi, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Immunology, 02 engineering and technology, Medical Biochemistry, Article, 03 medical and health sciences, Breast cancer, Immune system, Antigen, Tn antigen, medicine, lcsh:Science, Triple-negative breast cancer, Cancer, Multidisciplinary, biology, business.industry, 021001 nanoscience & nanotechnology, medicine.disease, 030104 developmental biology, Tumor progression, Cancer cell, biology.protein, Cancer research, lcsh:Q, Antibody, 0210 nano-technology, business, Vaccine |
| الوصف: | Summary The Tn antigen is a well-known tumor-associated carbohydrate determinant, often incorporated in glycopeptides to develop cancer vaccines. Herein, four copies of a conformationally constrained mimetic of the antigen TnThr (GalNAc-Thr) were conjugated to the adjuvant CRM197, a protein licensed for human use. The resulting vaccine candidate, mime[4]CRM elicited a robust immune response in a triple-negative breast cancer mouse model, correlated with high frequency of CD4+ T cells and low frequency of M2-type macrophages, which reduces tumor progression and lung metastasis growth. Mime[4]CRM-mediated activation of human dendritic cells is reported, and the proliferation of mime[4]CRM-specific T cells, in cancer tissue and peripheral blood of patients with breast cancer, is demonstrated. The locked conformation of the TnThr mimetic and a proper presentation on the surface of CRM197 may explain the binding of the conjugate to the anti-Tn antibody Tn218 and its efficacy to fight cancer cells in mice. Graphical Abstract Highlights • Structurally simple vaccine candidate reduces BC tumor size and delays lung metastasis • TnThr mimetic, fused to CRM197 adjuvant, is able to elicit T and B immune responses • TnThr mimetic-based vaccine candidate able to activate human DCs • The vaccine candidate recruits T helper CD4+ in the tumor microenvironment Medical Biochemistry; Immunology; Cancer |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1016/j.isci.2020.101250 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af94a039b5fcd44108e2d9a6f2d63dfcTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af94a039b5fcd44108e2d9a6f2d63dfc |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1016/j.isci.2020.101250 |
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