VEGI, a new member of the TNF family activates Nuclear Factor-κB and c-Jun N-terminal kinase and modulates cell growth
| العنوان: | VEGI, a new member of the TNF family activates Nuclear Factor-κB and c-Jun N-terminal kinase and modulates cell growth |
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| المؤلفون: | Reiner L. Gentz, Guo Liang Yu, Steve Ruben, Su Fang Chen, Jian Ni, Valsala Haridas, Ding Liu, Jeffrey Y. Su, Anju Shrivastava, Yansong Ni, Bharat B. Aggarwal |
| المصدر: | Oncogene. 18:6496-6504 |
| بيانات النشر: | Springer Science and Business Media LLC, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Tumor Necrosis Factor Ligand Superfamily Member 15, Cancer Research, medicine.medical_treatment, Biology, Receptors, Tumor Necrosis Factor, chemistry.chemical_compound, Genetics, medicine, Humans, Protein kinase A, Molecular Biology, Transcription factor, DNA Primers, Base Sequence, Tumor Necrosis Factor-alpha, c-jun, JNK Mitogen-Activated Protein Kinases, NF-kappa B, NF-κB, Molecular biology, Recombinant Proteins, Enzyme Activation, IκBα, Cytokine, chemistry, Tumor necrosis factor alpha, Mitogen-Activated Protein Kinases, Signal transduction, Cell Division, Protein Binding |
| الوصف: | Recently a new member of the human tumor necrosis factor (TNF) family named as VEGI was reported. However, very little is known about the biological activities displayed by this cytokine. In this report, we show that in myeloid cells VEGI activated the transcription factor kappa B (NF-kappa B) as determined by the electrophoretic mobility shift assay, induced degradation of I kappa B alpha, and nuclear translocation of p65 subunit of NF-kappa B. VEGI also activated NF-kappa B-dependent reporter gene expression. In addition, VEGI activated c-Jun N-terminal kinase. When examined for growth modulatory effects, VEGI inhibited the proliferation of breast carcinoma (MCF-7), epithelial (HeLa), and myeloid (U-937 and ML-1a) tumor cells; and activated caspase-3 leading to PARP cleavage. VEGI-induced cytotoxicity was potentiated by inhibitors of protein synthesis. VEGI also induced proliferation of normal human foreskin fibroblast cells. The activity of VEGI could neither be neutralized by antibodies against TNF, nor could it compete with TNF binding, indicating that the activity of VEGI is not due to TNF and it binds to a distinct receptor. These results suggest that VEGI, a new member of the TNF family, has a signaling pathway similar to TNF and is most likely a multifunctional cytokine. |
| تدمد: | 1476-5594 0950-9232 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af271793bd2fe4c747678a65faee59c7Test https://doi.org/10.1038/sj.onc.1203059Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af271793bd2fe4c747678a65faee59c7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765594 09509232 |
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