التفاصيل البيبلوغرافية
| العنوان: |
A subset of walnut allergic adults is sensitized to walnut 11S globulin Jug r 4 |
| المؤلفون: |
Kitty C.M. Verhoeckx, André C. Knulst, Marco Gaspari, W.M. Blom, Henderikus G. Otten, C. F. den Hartog Jager, Mark A. Blankestijn, G. A.H. de Jong, Geert F. Houben |
| المصدر: |
Clinical and Experimental Allergy, 48(9), 1206. Wiley-Blackwell |
| سنة النشر: |
2018 |
| مصطلحات موضوعية: |
0301 basic medicine, Male, Allergy, medicine.disease_cause, Immunoglobulin E, Mass Spectrometry, 11s globulin, 0302 clinical medicine, Allergen, immunoblot, Immunology and Allergy, Walnut allergy, Sensitization, Plant Proteins, Immunoassay, education.field_of_study, biology, Predictive value, medicine.anatomical_structure, Female, Nut Hypersensitivity, walnut allergy, Juglans, Adult, Population, Immunology, double blind placebo controlled food challenge, Cross Reactions, Immunoblot, Sensitivity and Specificity, 03 medical and health sciences, Young Adult, medicine, Double blind placebo controlled food challenge, Humans, Jug r 4, education, Skin Tests, Nutrition, business.industry, Plant Extracts, Antigens, Plant, medicine.disease, biology.organism_classification, 030104 developmental biology, 030228 respiratory system, biology.protein, business, Chromatography, Liquid |
| الوصف: |
Background: The role of sensitization to commercially available allergens of English walnut (Juglans regia) Jug r 1, 2 and 3 in walnut allergy has been previously investigated in walnut allergic adults and was unable to explain all cases of walnut allergy. Objectives: Identify recognized walnut allergens, other than the ones previously investigated (Jug r 1-3), in walnut allergic adults and determine the sensitization frequency and diagnostic value. Methods: Three different in-house walnut extracts were prepared and analysed on SDS-PAGE blots to identify allergenic walnut proteins. Immunoblots and immunoprecipitation, followed by LC-MS analysis, were performed to screen for, and confirm, IgE binding to walnut allergens in selected walnut allergic adults. In a cohort of 55 walnut challenged adults, including 33 allergic and 22 tolerant, sensitization to native and recombinant walnut allergen Jug r 4 was assessed using immunoblotting and immuno-line blot (EUROLINE), respectively. Results: Screening of sera of 8 walnut allergic adults identified Jug r 4 as an allergen in our population. In the total cohort of 55 subjects, 5 were positive for Jug r 4 on immunoblot and 10 on EUROLINE. All but one EUROLINE positive subject had a positive food challenge (sensitivity 27%, specificity 95%, PPV 90%, NPV 47%). All 5 subjects positive on immunoblot were also positive on EUROLINE. LC-MS analysis showed a lack of Jug r 4 in the ImmunoCAP extract. Co-sensitization to other 11S albumins (eg hazelnut Cor a 9) was common in Jug r 4 sensitized subjects, potentially due to cross-reactivity. Conclusions: Walnut 11S globulin Jug r 4 is a relevant minor allergen, recognized by 27% of walnut allergic adults. It has a high positive predictive value of 90% for walnut allergy. Specific IgE against Jug r 4 occurred mostly with concomitant sensitization to other walnut components, mainly Jug r 1. © 2018 The Authors. Clinical & Experimental Allergy Published by John Wiley & Sons Ltd |
| وصف الملف: |
application/pdf; image/pdf |
| اللغة: |
English |
| تدمد: |
0954-7894 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae8eb5189e831f2ee845fce17107139eTest
http://resolver.tudelft.nl/uuid:3f723994-0f0e-4f1c-8482-3ba8113cd27eTest |
| حقوق: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....ae8eb5189e831f2ee845fce17107139e |
| قاعدة البيانات: |
OpenAIRE |
