Evaluating the role of IL-11, a novel cytokine in the IL-6 family, in a mouse model of spinal cord injury
| العنوان: | Evaluating the role of IL-11, a novel cytokine in the IL-6 family, in a mouse model of spinal cord injury |
|---|---|
| المؤلفون: | Michael G. Fehlings, Dung H. Nguyen, Newton Cho, Donald R. Branch, Kajana Satkunendrarajah |
| المصدر: | Journal of Neuroinflammation Journal of Neuroinflammation, Vol 9, Iss 1, p 134 (2012) |
| بيانات النشر: | BioMed Central, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Cord, medicine.medical_treatment, mouse model, Immunology, Spinal cord injury, lcsh:RC346-429, immune response, 03 medical and health sciences, Cellular and Molecular Neuroscience, Mice, 0302 clinical medicine, gp130 receptor, medicine, Cytokine Receptor gp130, Animals, locomotor recovery, Interleukin-11 Receptor alpha Subunit, lcsh:Neurology. Diseases of the nervous system, Spinal Cord Injuries, 030304 developmental biology, Mice, Knockout, 0303 health sciences, business.industry, Interleukin-6, General Neuroscience, Research, Glycoprotein 130, Spinal cord, medicine.disease, electrophysiology, Interleukin-11, 3. Good health, Up-Regulation, Mice, Inbred C57BL, Disease Models, Animal, Cytokine, medicine.anatomical_structure, Neurology, Motor Skills, Multigene Family, Knockout mouse, IL-11, Signal transduction, business, Leukemia inhibitory factor, 030217 neurology & neurosurgery |
| الوصف: | Background Spinal cord injury (SCI) is a devastating condition with substantial functional and social morbidity. Previous research has established that the neuroinflammatory response plays a significant role in cord damage post-SCI. However, global immunosuppressive therapies have demonstrated mixed results. As a result, more specific therapies modulating inflammation after injury are needed. In this regard, research into cytokine signaling has demonstrated that cytokines of the gp130 family including IL-6 and leukemia inhibitory factor (LIF) play key roles in mediating damage to the spinal cord. Since members of the gp130 family all share a common signal transduction pathway via the JAK/STAT system, we performed the first study of a relatively new member of the gp130 family, IL-11, in SCI. Methods A validated clip-compression mouse model of SCI was used to assess for temporal changes in expression of IL-11 and its receptor, IL-11Rα, post-SCI. To elucidate the role of IL-II in the pathophysiology of SCI, we compared differences in locomotor recovery (Basso Mouse Score; CatWalk), electrophysiological spinal cord signaling, histopathology, and the acute inflammatory neutrophil response in IL-11Rα knockouts with littermate wild-type C57BL/6 mice. Results We found an increase in gene expression of IL-11 in the spinal cord to a peak at twenty-four hours post-SCI with increases in IL-11Rα gene expression, peaking at seven days post-SCI. In spite of clear changes in the temporal expression of both IL-11 and its receptor, we found that there were no significant differences in motor function, electrophysiological signaling, histopathology, or neutrophil infiltration into the spinal cord between wild-type and knockout mice. Conclusions This is the first study to address IL-11 in SCI. This study provides evidence that IL-11 signaling may not play as significant a role in SCI as other gp130 cytokines, which will ideally guide future therapy design and the signaling pathways those therapies target. |
| اللغة: | English |
| تدمد: | 1742-2094 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae65a221991e106f64df0d9b71736478Test http://europepmc.org/articles/PMC3410772Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ae65a221991e106f64df0d9b71736478 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17422094 |
|---|