pORE: a modular binary vector series suited for both monocot and dicot plant transformation
| العنوان: | pORE: a modular binary vector series suited for both monocot and dicot plant transformation |
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| المؤلفون: | Catherine Coutu, Brian Miki, Dwayne D. Hegedus, Jim Brandle, John Simmonds, Daniel C. W. Brown, Kirk Brown |
| المصدر: | Transgenic Research. 16:771-781 |
| بيانات النشر: | Springer Science and Business Media LLC, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | FLP-FRT recombination, Genetic Vectors, Molecular Sequence Data, Arabidopsis, Biology, Origin of replication, Zea mays, Transformation, Genetic, Plasmid, Genetics, Multiple cloning site, Selectable marker, Reporter gene, ColE1, Brassica napus, fungi, Peas, Plants, Genetically Modified, Transformation (genetics), Agrobacterium tumefaciens, Animal Science and Zoology, Soybeans, Agronomy and Crop Science, Plasmids, Biotechnology |
| الوصف: | We present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The vector size has been minimized by eliminating all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently. The smallest of the vector series is 6.3 kb and possesses an extensive multiple cloning site with 21 unique restriction endonuclease sites that are compatible with common cloning, protein expression, yeast two-hybrid and other binary vectors. The T-DNA region was engineered using a synthetic designer oligonucleotide resulting in an entirely modular system whereby any vector element can be independently exchanged. The high copy number ColE1 origin of replication has been included to enhance plasmid yield in Escherichia coli. FRT recombination sites flank the selectable marker cassette regions and allow for in planta excision by FLP recombinase. The pORE series consists of three basic types; an ‘open’ set for general plant transformation, a ‘reporter’ set for promoter analysis and an ‘expression’ set for constitutive expression of transgenes. The sets comprise various combinations of promoters (P HPL, P ENTCUP2 and P TAPADH), selectable markers (nptII and pat) and reporter genes (gusA and smgfp). |
| تدمد: | 1573-9368 0962-8819 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad17cd1f54a4f5ca6a10ff094d056f70Test https://doi.org/10.1007/s11248-007-9066-2Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ad17cd1f54a4f5ca6a10ff094d056f70 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15739368 09628819 |
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