A novel spliced gene in alcelaphine herpesvirus 1 encodes a glycoprotein which is secreted in vitro
| العنوان: | A novel spliced gene in alcelaphine herpesvirus 1 encodes a glycoprotein which is secreted in vitro |
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| المؤلفون: | James P. Stewart, Ann Percival, George C. Russell, Mark P. Dagleish, David M. Haig, David Deane, Helen Todd |
| المصدر: | Journal of General Virology. 94:2515-2523 |
| بيانات النشر: | Microbiology Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Sequence analysis, Molecular Sequence Data, DNA, Recombinant, Cattle Diseases, Biology, Kidney, Gammaherpesvirinae, Virology, Complementary DNA, Animals, Coding region, Amino Acid Sequence, Gene, Peptide sequence, Glycoproteins, Genetics, chemistry.chemical_classification, RNA, Herpesviridae Infections, Sequence Analysis, DNA, Fusion protein, Molecular biology, chemistry, Malignant Catarrh, Cattle, Glycoprotein |
| الوصف: | Herpesviruses often contain cryptic, spliced genes that are not obvious from the initial in silico annotation. Alcelaphine herpesvirus 1 (AlHV-1) contains 72 annotated ORFs but there are also a number of gaps between these that may have protein-coding potential. Comparative analysis of coding potential between AlHV-1 and the related ovine herpesvirus 2 (OvHV-2) revealed a putative novel spliced gene that we have termed A9.5. Analysis of cDNA clones from AlHV-1-infected cells revealed three overlapping clones corresponding to A9.5 and the coding sequence was confirmed by reverse transcription PCR of RNA from AlHV-1-infected cattle tissues. The A9.5 gene was predicted to encode a secreted glycoprotein with molecular mass 19 kDa. Empirical analysis showed that a recombinant haemagglutinin-tagged A9.5 fusion protein was secreted from transfected cells and had a molecular mass of 45 kDa, which was reduced to 20 kDa by endoglycosidase F treatment, confirming that A9.5 was a secreted glycoprotein. In situ RNA hybridization showed that A9.5 was expressed in cells associated with malignant catarrhal fever (MCF) lesions in infected cattle. Detailed analysis of the available OvHV-2 sequences revealed an homologous gene (Ov9.5) with conserved splicing signals and predicted amino acid sequence features in both sequenced isolates of this related virus. We have therefore identified a novel spliced gene in two related macaviruses that is expressed in MCF lesions. Future work will determine its importance for the pathogenesis of disease. |
| تدمد: | 1465-2099 0022-1317 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acec04a924f98d8cfa07933ba6849ac4Test https://doi.org/10.1099/vir.0.055673-0Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....acec04a924f98d8cfa07933ba6849ac4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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