Histones H1 and H4 of surface-spread meiotic chromosomes
| العنوان: | Histones H1 and H4 of surface-spread meiotic chromosomes |
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| المؤلفون: | Peter B. Moens |
| المصدر: | Chromosoma. 104:169-174 |
| بيانات النشر: | Springer Science and Business Media LLC, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Male, Transcription, Genetic, Euchromatin, Gene Expression, Biology, Chromosomes, Histones, Histone H4, Mice, Histone H1, Heterochromatin, Testis, Histone methylation, Histone H2A, Genetics, Animals, Histone code, Nucleosome, Phosphorylation, Fluorescent Antibody Technique, Indirect, Metaphase, Genetics (clinical), Synaptonemal Complex, Acetylation, Molecular biology, Chromatin, Cell biology, Meiosis, Protein Biosynthesis, Heterochromatin protein 1 |
| الوصف: | The chromatin conformation of somatic and meiotic chromosomes is, at least in part, a function of electrostatic nucleosome interactions that are mediated by transient acetylation of the histone H4 N-terminal domain and phosphorylation of histone H1. The distribution of those histones in the chromatin of meiotic chromosomes is reported here. Antibodies to testis-specific histone 1, H1t, detect H1t in the chromatin of mouse meiotic prophase chromosomes only after synapsis and synaptonemal complex (SC) assembly is completed and before core separation is initiated. The H1t protein is evenly distributed over euchromatin, heterochromatin and the SC. Antibodies to acetylated lysine residues 5, 12 or 16 of histone H4, indicate that the euchromatin is more acetylated than the centromeric heterochromatin. The pattern is most pronounced for acetylated residue 5 and least for 16. Antibodies to phosphorylated H1 epitopes do not react with chromatin but, instead, recognize the chromosome cores and SCs. Possibly these are not phosphorylated histone H1 epitopes, but SC proteins with similar potentially phosphorylatable sequences such as KTPTK of the synaptic protein Syn1. |
| تدمد: | 1432-0886 0009-5915 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab8e42f71a931fe42b3331f008b15d13Test https://doi.org/10.1007/bf00352181Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ab8e42f71a931fe42b3331f008b15d13 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320886 00095915 |
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