Pivotal role of matrix metalloproteinase 13 in extracellular matrix turnover in idiopathic pulmonary fibrosis
| العنوان: | Pivotal role of matrix metalloproteinase 13 in extracellular matrix turnover in idiopathic pulmonary fibrosis |
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| المؤلفون: | Takayuki Shiomi, Yasunori Okada, Clemens Ruppert, Werner Seeger, Andreas Günther, György Lang, Jeanine D'Armiento, Takwi Nkyimbeng, Bhola K. Dahal |
| المصدر: | PLoS ONE, Vol 8, Iss 9, p e73279 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Pathology, lcsh:Medicine, Matrix metalloproteinase, Extracellular matrix, Mice, chemistry.chemical_compound, Idiopathic pulmonary fibrosis, 0302 clinical medicine, Fibrosis, Pulmonary fibrosis, lcsh:Science, Lung, 0303 health sciences, Multidisciplinary, Tissue Inhibitor of Metalloproteinases, Middle Aged, respiratory system, Tissue Donors, Extracellular Matrix, Up-Regulation, 3. Good health, Hydroxyproline, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Collagen, Matrix Metalloproteinase 1, medicine.symptom, Research Article, Adult, medicine.medical_specialty, Inflammation, Bleomycin, 03 medical and health sciences, Matrix Metalloproteinase 13, medicine, Animals, Humans, 030304 developmental biology, business.industry, lcsh:R, medicine.disease, Idiopathic Pulmonary Fibrosis, respiratory tract diseases, chemistry, Case-Control Studies, Proteolysis, lcsh:Q, business |
| الوصف: | Rationale Idiopathic pulmonary fibrosis (IPF) is a fatal disease characterized by excessive deposition of extracellular matrix (ECM). Objectives We investigated the regulation of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in lung fibrosis. Methods MMP and TIMP expression, collagenolytic activity and collagen content was assessed in IPF (n=16) versus donor (n=6) lung homogenates and accomplished by in-situ-zymography for gelatinolytic and collagenolytic activities, combined with MMP antigen detection. Role of MMP13 was assessed employing the bleomycin model of lung fibrosis in MMP-13-/- versus wild-type mice. Measurements and Main Results In IPF, MMPs-1, 2, 7, 9 and 13, but not MMP-8, were significantly upregulated, whereas none of the TIMPs (1–4) were significantly altered. Collagen content was slightly increased and collagenolytic activity was most prominent in the airways and co-localized with MMP-13. We observed an exaggerated early inflammatory response and an augmented lung fibrosis in bleomycin-challenged MMP-13-/- versus wild-type mice, with elevated lung collagen content 28d after bleomycin challenge in the MMP-13-/- mice. Conclusions Our data suggest that i) collagen deposition in IPF lungs is not primarily due to excessive TIMP production, but rather due to overwhelming ECM production in face of an overall increased, but spatially imbalanced collagenolytic activity, ii) preferential distribution of collagenolytic activity, largely MMP-13, in the airways offers an explanation for the development of honeycomb cysts and iii) despite an overall increase in inflammatory cell content the presence of MMP-13 seems to limit the overall extent of ECM deposition in lung fibrosis. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa39d7bd70c09ca52ff89d14f940de25Test http://europepmc.org/articles/PMC3759404?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....aa39d7bd70c09ca52ff89d14f940de25 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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