Comparison between two inhibin B ELISA assays in 46,XY testicular disorders of sex development (DSD) with normal testosterone secretion
| العنوان: | Comparison between two inhibin B ELISA assays in 46,XY testicular disorders of sex development (DSD) with normal testosterone secretion |
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| المؤلفون: | Gil Guerra-Júnior, André Moreno Morcillo, Guilherme Guaragna-Filho, Antonio Ramos Calixto, Georgette Beatriz De Paula, Laurione Cândido de Oliveira, Maricilda Palandi de Mello, Andréa Trevas Maciel-Guerra |
| المصدر: | Journal of Pediatric Endocrinology and Metabolism. 31:191-194 |
| بيانات النشر: | Walter de Gruyter GmbH, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Male, Steroid Metabolism, Inborn Errors, medicine.medical_specialty, Outpatient Clinics, Hospital, Adolescent, Testicular Disorder, Endocrinology, Diabetes and Metabolism, Karyotype, Enzyme-Linked Immunosorbent Assay, 030209 endocrinology & metabolism, Steroidogenic Factor 1, Severity of Illness Index, Diagnosis, Differential, Hospitals, University, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, 3-Oxo-5-alpha-Steroid 4-Dehydrogenase, Internal medicine, Testis, medicine, Humans, Testosterone, Disorders of sex development, Child, Partial androgen insensitivity syndrome, Inhibin-beta Subunits, Hypospadias, Disorder of Sex Development, 46,XY, 030219 obstetrics & reproductive medicine, business.industry, Membrane Proteins, Reproducibility of Results, Androgen-Insensitivity Syndrome, medicine.disease, Sertoli cell, Confidence interval, medicine.anatomical_structure, Receptors, Androgen, Child, Preschool, SRD5A2, Pediatrics, Perinatology and Child Health, business, Hormone |
| الوصف: | Background: Inhibin B is a hormone produced by the Sertoli cells that can provide important information for the investigation of disorders of sex development (DSD) with 46,XY karyotype. The aim of this study is to compare two enzyme-linked immunosorbent assay (ELISA) assays for dosage of serum inhibin B in patients with 46,XY DSD with normal testosterone secretion. Methods: Twenty-nine patients with 46,XY DSD and normal testosterone secretion (partial androgen insensitivity syndrome [PAIS] [n=8]; 5α-reductase deficiency [n=7] and idiopathic 46,XY DSD [n=14]) were included. Molecular analysis of the AR and SRD5A2 genes were performed in all patients and the NR5A1 gene analysis in the idiopathic group. Measurements of inhibin B were performed by two second-generation ELISA assays (Beckman-Coulter and AnshLabs). Assays were compared using the interclass correlation coefficient (ICC) and the Bland-Altman method. Results: ICC was 0.915 [95% confidence interval (CI): 0.828–0.959], however, a discrepancy was observed between trials, which is more evident among higher values when analyzed by the Bland-Altman method. Conclusions: It is recommended to perform the inhibin B measurement always using the same ELISA kit when several evaluations are required for a specific patient. |
| تدمد: | 2191-0251 0334-018X 2017-0351 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa34080f4a9bf20cee6b60e89067ada6Test https://doi.org/10.1515/jpem-2017-0351Test |
| رقم الانضمام: | edsair.doi.dedup.....aa34080f4a9bf20cee6b60e89067ada6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21910251 0334018X 20170351 |
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