Gadolinium-enhanced cardiac MR exams of human subjects are associated with significant increases in the DNA repair marker 53BP1, but not the damage marker γH2AX
| العنوان: | Gadolinium-enhanced cardiac MR exams of human subjects are associated with significant increases in the DNA repair marker 53BP1, but not the damage marker γH2AX |
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| المؤلفون: | David F. Kallmes, Sylvain V. Costes, Tamara M. Hudson, Dana Schroeder, Jennifer S. McDonald, Philip M. Young, Jacob B. Ekins, Anthony S. Tin, Robert J. McDonald, Aiming Lu, Ramanathan Kadirvel, Scott H. Kaufmann, Kevin M. Kallmes |
| المصدر: | PLoS ONE, Vol 13, Iss 1, p e0190890 (2018) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Pathology, DNA Repair, Physiology, medicine.medical_treatment, lcsh:Medicine, Gadolinium, 030204 cardiovascular system & hematology, Biochemistry, Diagnostic Radiology, Histones, White Blood Cells, 0302 clinical medicine, Animal Cells, Medicine and Health Sciences, Lymphocytes, Prospective Studies, Prospective cohort study, lcsh:Science, Multidisciplinary, medicine.diagnostic_test, Radiology and Imaging, Heart, Middle Aged, Magnetic Resonance Imaging, Body Fluids, Nucleic acids, Chemistry, Blood, Physical Sciences, Female, Cellular Types, Anatomy, Cardiomyopathies, Tumor Suppressor p53-Binding Protein 1, Research Article, Chemical Elements, Adult, medicine.medical_specialty, DNA damage, Imaging Techniques, Immune Cells, Immunology, Cardiology, Malignancy, Research and Analysis Methods, Drug Absorption, Peripheral blood mononuclear cell, 03 medical and health sciences, Diagnostic Medicine, medicine, Genetics, Humans, Clinical significance, Pharmacokinetics, Aged, Retrospective Studies, Pharmacology, Chemotherapy, Blood Cells, Biology and life sciences, business.industry, lcsh:R, Correction, Magnetic resonance imaging, DNA, Cell Biology, medicine.disease, Radiation therapy, 030104 developmental biology, lcsh:Q, business, Biomarkers, DNA Damage |
| الوصف: | Magnetic resonance imaging is considered low risk, yet recent studies have raised a concern of potential damage to DNA in peripheral blood leukocytes. This prospective Institutional Review Board-approved study examined potential double-strand DNA damage by analyzing changes in the DNA damage and repair markers γH2AX and 53BP1 in patients who underwent a 1.5 T gadolinium-enhanced cardiac magnetic resonance (MR) exam. Sixty patients were enrolled (median age 55 years, 39 males). Patients with history of malignancy or who were receiving chemotherapy, radiation therapy, or steroids were excluded. MR sequence data were recorded and blood samples obtained immediately before and after MR exposure. An automated immunofluorescence assay quantified γH2AX or 53BP1 foci number in isolated peripheral blood mononuclear cells. Changes in foci number were analyzed using the Wilcoxon signed-rank test. Clinical and MR procedural characteristics were compared between patients who had a >10% increase in γH2AX or 53BP1 foci numbers and patients who did not. The number of γH2AX foci did not significantly change following cardiac MR (median foci per cell pre-MR = 0.11, post-MR = 0.11, p = .90), but the number of 53BP1 foci significantly increased following MR (median foci per cell pre-MR = 0.46, post-MR = 0.54, p = .0140). Clinical and MR characteristics did not differ significantly between patients who had at least a 10% increase in foci per cell and those who did not. We conclude that MR exposure leads to a small (median 25%) increase in 53BP1 foci, however the clinical relevance of this increase is unknown and may be attributable to normal variation instead of MR exposure. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9f306e02fb700f124b332c39429b22bTest http://europepmc.org/articles/PMC5757995?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a9f306e02fb700f124b332c39429b22b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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