Cross-sectional prevalence study of MERS-CoV in local and imported dromedary camels in Saudi Arabia, 2016-2018
| العنوان: | Cross-sectional prevalence study of MERS-CoV in local and imported dromedary camels in Saudi Arabia, 2016-2018 |
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| المؤلفون: | Sherif A. El-Kafrawy, Steve Harakeh, Ahmed A. Mirza, Mohammed A. Alsaadi, Esam I. Azhar, Anwar M. Hashem, Nabil Amor, Ahmed M. Tolah, Ahmed M. Hassan, Saad B. Al Masaudi, Ghaleb A. Alsaaidi, Abdulaziz N. Alagaili, Abdulrahman A. Alzahrani |
| المصدر: | PLoS ONE PLoS ONE, Vol 15, Iss 5, p e0232790 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, RNA viruses, Veterinary medicine, Pulmonology, Cross-sectional study, Coronaviruses, viruses, Artificial Gene Amplification and Extension, Antibodies, Viral, Pathology and Laboratory Medicine, Polymerase Chain Reaction, Geographical locations, Sudan, 0302 clinical medicine, Camels, Seroepidemiologic Studies, Epidemiology, Prevalence, Medicine and Health Sciences, 030212 general & internal medicine, Mammals, Multidisciplinary, Eukaryota, Medical Microbiology, Viral Pathogens, Vertebrates, Viruses, Middle East Respiratory Syndrome Coronavirus, Medicine, Djibouti, RNA, Viral, Female, Detection rate, Pathogens, Coronavirus Infections, Viral load, Research Article, 2019-20 coronavirus outbreak, medicine.medical_specialty, endocrine system, Viral Release, Camelus, Asia, Science, 030231 tropical medicine, Saudi Arabia, Biology, Research and Analysis Methods, Microbiology, 03 medical and health sciences, Extraction techniques, Virology, parasitic diseases, medicine, Seroprevalence, Animals, Viral rna, Molecular Biology Techniques, Microbial Pathogens, Molecular Biology, Disease Reservoirs, Organisms, Biology and Life Sciences, Reverse Transcriptase-Polymerase Chain Reaction, Antibodies, Neutralizing, RNA extraction, Cross-Sectional Studies, Amniotes, Respiratory Infections, Africa, Herd, People and places, Viral Transmission and Infection |
| الوصف: | The Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) is an endemic virus in dromedaries. Annually, Saudi Arabia imports thousands of camels from the Horn of Africa, yet the epidemiology of MERS-CoV in these animals is largely unknown. Here, MERS-CoV prevalence was compared in imported African camels and their local counterparts. A total of 1399 paired sera and nasal swabs were collected from camels between 2016 and 2018. Imported animals from Sudan (n = 829) and Djibouti (n = 328) were sampled on incoming ships at Jeddah Islamic seaport before unloading, and local camels were sampled from Jeddah (n = 242). Samples were screened for neutralizing antibodies (nAbs) and MERS-CoV viral RNA. The overall seroprevalence was 92.7% and RNA detection rate was 17.2%. Imported camels had higher seroprevalence compared to resident herds (93.8% vs 87.6%, p 87% of the RNA positive animals, increased with age and was sex-dependent. Importantly, reduced viral RNA load was positively correlated with nAb titers. Our data confirm the widespread of MERS-CoV in imported and domestic camels in Saudi Arabia and highlight the need for continuous active surveillance and better prevention measures. Further studies are also warranted to understand camels correlates of protection for proper vaccine development. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8fb1bda08abeed47277c415a01ae34cTest https://pubmed.ncbi.nlm.nih.gov/32453746Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a8fb1bda08abeed47277c415a01ae34c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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