High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer
| العنوان: | High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer |
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| المؤلفون: | Anu Puri, Masaki Terabe, Peter L. Choyke, Carley D. Ross, Jay A. Berzofsky, Aparna H. Kesarwala, James C. S. Wood, Jane B. Trepel, Stephen P. Perfetto, Joshua A. Welsh, John Tigges, Hisataka Kobayashi, William G. Telford, Marjorie Robert-Guroff, Alan Dean, Thorsten Demberg, Jennifer Jones, Bryant C. Yung, David R. Parks, Aliaksander Kachynski, Jonathan Van Dyke, Xiaoyuan Chen, Marty Bigos, Ariel Rosner, Thomas Musich, Gordon J. Freeman, Aizea Morales-Kastresana, Susan J. Knox, Edward J. Felton, Lise Pasquet, W. Roy Overton, Vasilis Toxavidis, Peter Guion, Katherine McKinnon, Veena Kapoor, Ionita Ghiran |
| المصدر: | Journal of Extracellular Vesicles Journal of extracellular vesicles, vol 8, iss 1 Journal of Extracellular Vesicles, Vol 8, Iss 1 (2019) |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Nanofacs, phenotyping, Histology, Bioengineering, Flow cytometry, Background noise, 03 medical and health sciences, 0302 clinical medicine, medicine, Nanotechnology, lcsh:QH573-671, Tropism, Cancer, medicine.diagnostic_test, lcsh:Cytology, Chemistry, flow cytometry, Vesicle, Sorting, RNA, Cell Biology, Fluorescence, Microvesicles, 3. Good health, 030104 developmental biology, 030220 oncology & carcinogenesis, Biophysics, Biochemistry and Cell Biology, extracellular vesicles, sorting, Research Article |
| الوصف: | Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of conventional flow cytometers due to the overlap of particle-associated scattered light signals with the detection of background instrument noise from diffusely scattered light. To identify, sort, and study distinct subsets of EVs and other nanoparticles, as individual particles, we developed nanoscale Fluorescence Analysis and Cytometric Sorting (nanoFACS) methods to maximise information and material that can be obtained with high speed, high resolution flow cytometers. This nanoFACS method requires analysis of the instrument background noise (herein defined as the “reference noise”). With these methods, we demonstrate detection of tumour cell-derived EVs with specific tumour antigens using both fluorescence and scattered light parameters. We further validated the performance of nanoFACS by sorting two distinct HIV strains to >95% purity and confirmed the viability (infectivity) and molecular specificity (specific cell tropism) of biological nanomaterials sorted with nanoFACS. This nanoFACS method provides a unique way to analyse and sort functional EV- and viral-subsets with preservation of vesicular structure, surface protein specificity and RNA cargo activity. |
| وصف الملف: | application/pdf |
| تدمد: | 2001-3078 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7b37c35b084de708e93d07459cc6c84Test https://doi.org/10.1080/20013078.2019.1597603Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a7b37c35b084de708e93d07459cc6c84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20013078 |
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