Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis
| العنوان: | Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis |
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| المؤلفون: | Francis A. Barr, John M. Lucocq, James G. Pryde, Philip G. Woodman, Martin Lowe, Jon D. Lane, Victoria J. Allan |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Green Fluorescent Proteins, Golgi Apparatus, Apoptosis, Caspase 3, Golgi reassembly, Cleavage (embryo), Autoantigens, Article, Amino Acid Chloromethyl Ketones, Golgi apparatus, apoptosis, GRASP65, Golgi structure, caspase, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Humans, Enzyme Inhibitors, Caspase, Glycoproteins, 030304 developmental biology, 0303 health sciences, Membrane Glycoproteins, Microscopy, Video, biology, Golgi Matrix Proteins, Membrane Proteins, Proteins, Cell Biology, Cell biology, Luminescent Proteins, Microscopy, Electron, Membrane, Microscopy, Fluorescence, Membrane protein, Caspases, biology.protein, symbols, N-Acetylgalactosaminyltransferases, Oligopeptides, 030217 neurology & neurosurgery, Cytokinesis, HeLa Cells, Peptide Hydrolases |
| الوصف: | The mammalian Golgi complex is comprised of a ribbon of stacked cisternal membranes often located in the pericentriolar region of the cell. Here, we report that during apoptosis the Golgi ribbon is fragmented into dispersed clusters of tubulo-vesicular membranes. We have found that fragmentation is caspase dependent and identified GRASP65 (Golgi reassembly and stacking protein of 65 kD) as a novel caspase substrate. GRASP65 is cleaved specifically by caspase-3 at conserved sites in its membrane distal COOH terminus at an early stage of the execution phase. Expression of a caspase-resistant form of GRASP65 partially preserved cisternal stacking and inhibited breakdown of the Golgi ribbon in apoptotic cells. Our results suggest that GRASP65 is an important structural component required for maintenance of Golgi apparatus integrity. |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a595f72b482f94b43671a26a5175df3cTest https://doi.org/10.1083/jcb.200110007Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a595f72b482f94b43671a26a5175df3c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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