Proteomic profiling of peripheral blood mononuclear cells isolated from patients with tuberculosis and diabetes copathogenesis - A pilot study
| العنوان: | Proteomic profiling of peripheral blood mononuclear cells isolated from patients with tuberculosis and diabetes copathogenesis - A pilot study |
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| المؤلفون: | Sadhna Sharma, S. R. Bakshi, Sanjay Kumar Bhadada, Indu Verma, H. C. Joshi, Jyoti Kundu |
| المصدر: | PLoS ONE PLoS ONE, Vol 15, Iss 11, p e0233326 (2020) |
| بيانات النشر: | Public Library of Science, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Bacterial Diseases, Proteomics, Proteome, Protein Expression, Gene Expression, Pilot Projects, Biochemistry, SH3 domain, 0302 clinical medicine, Medical Conditions, Endocrinology, Medicine and Health Sciences, Diabetes diagnosis and management, Multidisciplinary, Middle Aged, Actinobacteria, Infectious Diseases, Medicine, Female, Research Article, Tuberculosis, HbA1c, Endocrine Disorders, Science, Protein subunit, PDZ domain, Biology, Research and Analysis Methods, Peripheral blood mononuclear cell, S100A8, 03 medical and health sciences, DNA-binding proteins, medicine, Diabetes Mellitus, Gene Expression and Vector Techniques, Genetics, Humans, Gene Regulation, Hemoglobin, Molecular Biology Techniques, Molecular Biology, Molecular Biology Assays and Analysis Techniques, Bacteria, business.industry, Organisms, Biology and Life Sciences, Proteins, medicine.disease, Tropical Diseases, Molecular biology, Diagnostic medicine, Regulatory Proteins, Cytoskeletal Proteins, 030104 developmental biology, Metabolic Disorders, Case-Control Studies, Leukocytes, Mononuclear, Rab, business, 030217 neurology & neurosurgery, Mycobacterium Tuberculosis, Biomarkers, Transcription Factors |
| الوصف: | Background Diabetes is an important risk factor for developing tuberculosis. This association leads to exacerbation of tuberculosis symptoms and delayed treatment of both the diseases. Molecular mechanism and biomarkers/drug targets related to copathogenesis of tuberculosis and diabetes are still poorly understood. In this study, proteomics based 2D-MALDI/MS approach was employed to identify host signature proteins which are altered during copathogenesis of tuberculosis and diabetes. Methods Comparative proteome of human peripheral blood mononuclear cells (PBMCs) from healthy controls, tuberculosis and diabetes patients in comparison to comorbid diabetes and tuberculosis patients was analyzed. Gel based proteomics approach followed by in gel trypsin digestion and peptide identification by mass spectrometry was used for signature protein identification. Results Total of 18 protein spots with differential expression in tuberculosis and diabetes copathogenesis (TBDM) patients in comparison to other groups were identified. These proteins belonged to four functional categories i.e. structural, cell cycle/growth regulation, signaling and intermediary metabolism. These include Vimentin, tubulin beta chain protein, Actin related protein 2/3 complex subunit 2, coffilin 1 (Structural), PDZ LIM domain protein, Rho-GDP dissociation inhibitor, Ras related protein Rab (signaling), superoxide dismutase, dCTPpyrophosphatase 1, Transcription initiation factor TFIID subunit 12, three isoforms of Peptidylprolylcis-trans isomerase A, SH3 domain containing protein (metabolism), three isoforms of Protein S100A9 and S100A8 (cell cycle progression/growth regulation). Conclusion Proteins identified to be differentially expressed in TBDM patient can act as potent biomarkers and as predictors for copathogenesis of tuberculosis and diabetes. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a55ad1c1774332f245c4eb45933f09a0Test http://europepmc.org/articles/PMC7647457Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a55ad1c1774332f245c4eb45933f09a0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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