Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT
| العنوان: | Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT |
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| المؤلفون: | Maksim A Yakovlev, Dilworth Y. Parkinson, Keith C. Cheng, Yifu Ding, Spencer R Katz, Daniel J. Vanselow, Khai C. Ang, Victor A. Canfield, Yuxin Wang, Alex Y. Lin |
| المصدر: | eLife eLife, Vol 10 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Silver Staining, QH301-705.5, Science, ved/biology.organism_classification_rank.species, Mutant, Human skin, Context (language use), micro-CT, General Biochemistry, Genetics and Molecular Biology, Imaging, Silver stain, Melanin, developmental biology, Imaging, Three-Dimensional, 3D imaging, cell biology, Animals, pigmentation, Biology (General), Model organism, whole-organism phenomics, Zebrafish, Melanins, General Immunology and Microbiology, biology, ved/biology, Chemistry, General Neuroscience, General Medicine, X-Ray Microtomography, Cell Biology, Zebrafish Proteins, biology.organism_classification, zebrafish, Chromatophore, Phenotype, Cell biology, melanin, Biochemistry, Three-Dimensional, Medicine, Biomedical Imaging, Biochemistry and Cell Biology, Research Advance, Whole Organism, Developmental Biology |
| الوصف: | We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables. |
| وصف الملف: | application/pdf |
| تدمد: | 2050-084X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a530c7306dc5b071f862f7ab690c30fdTest https://pubmed.ncbi.nlm.nih.gov/34528510Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a530c7306dc5b071f862f7ab690c30fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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