Molecular adaptation and salt stress response of Halobacterium salinarum cells revealed by neutron spectroscopy
| العنوان: | Molecular adaptation and salt stress response of Halobacterium salinarum cells revealed by neutron spectroscopy |
|---|---|
| المؤلفون: | Pierre Vauclare, Judith Peters, Nicolas Martinez, Vincent Marty, Frank Gabel, Marion Jasnin, E. Fabiani, G. Zaccai, Bruno Franzetti |
| المساهمون: | Thomas, Frank, Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Laue-Langevin (ILL), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), ILL |
| المصدر: | Extremophiles Extremophiles, 2015, 19 (6), pp.1099-107 Extremophiles, Springer Verlag, 2015, 19 (6), pp.1099-107 |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Halobacterium salinarum, Molar concentration, biology, Water activity, Proteome, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Archaeal Proteins, General Medicine, Salt Tolerance, Protein aggregation, biology.organism_classification, Microbiology, Enzyme structure, Halophile, Biochemistry, Stress, Physiological, Potassium, Molecular Medicine, Osmotic pressure, Denaturation (biochemistry) |
| الوصف: | International audience; Halobacterium salinarum is an extreme halophile archaeon with an absolute requirement for a multimolar salt environment. It accumulates molar concentrations of KCl in the cytosol to counterbalance the external osmotic pressure imposed by the molar NaCl. As a consequence, cytosolic proteins are permanently exposed to low water activity and highly ionic conditions. In non-adapted systems, such conditions would promote protein aggregation, precipitation, and denaturation. In contrast, in vitro studies showed that proteins from extreme halophilic cells are themselves obligate halophiles. In this paper, adaptation via dynamics to low-salt stress in H. salinarum cells was measured by neutron scattering experiments coupled with microbiological characterization. The molecular dynamic properties of a proteome represent a good indicator for environmental adaptation and the neutron/microbiology approach has been shown to be well tailored to characterize these modifications. In their natural setting, halophilic organisms often have to face important variations in environmental salt concentration. The results showed deleterious effects already occur in the H. salinarum proteome, even when the external salt concentration is still relatively high, suggesting the onset of survival mechanisms quite early when the environmental salt concentration decreases. |
| اللغة: | English |
| تدمد: | 1431-0651 1433-4909 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3fd8bb8670f46d6936df7ca9be88f2aTest https://hal.univ-grenoble-alpes.fr/hal-01234062Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....a3fd8bb8670f46d6936df7ca9be88f2a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14310651 14334909 |
|---|