ThymicPeptides Reverse Immune Exhaustion in Patients with Reactivated Human Alphaherpesvirus1 Infections
| العنوان: | ThymicPeptides Reverse Immune Exhaustion in Patients with Reactivated Human Alphaherpesvirus1 Infections |
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| المؤلفون: | Jacek Roliński, Anna Hymos, Maria Klatka, Ewelina Grywalska, Janusz Klatka, Izabela Korona-Glowniak |
| المصدر: | International Journal of Molecular Sciences Volume 21 Issue 7 International Journal of Molecular Sciences, Vol 21, Iss 2379, p 2379 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, thymic peptides, CD3, Programmed Cell Death 1 Receptor, Herpesvirus 1, Human, Peripheral blood mononuclear cell, Antiviral Agents, Thymus Extracts, Catalysis, Article, Proinflammatory cytokine, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, 0302 clinical medicine, Immune system, Adjuvants, Immunologic, Medicine, Humans, Interferon gamma, IL-2 receptor, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, immune exhaustion, Spectroscopy, Cells, Cultured, biology, business.industry, Organic Chemistry, Interleukin, Herpes Simplex, General Medicine, programmed cell death protein 1, Middle Aged, Computer Science Applications, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Immunology, biology.protein, Latent Infection, Cytokines, Female, business, CD8, 030215 immunology, medicine.drug |
| الوصف: | Recurrent infection with human alphaherpesvirus 1 (HHV-1) may be associated with immune exhaustion that impairs virus elimination. Thymic peptides enhance immune function and thus could overcome immune exhaustion. In this study, we investigated whether reactivation of herpes infections was associated with immune exhaustion. Moreover, we examined the impact of treatment with thymostimulin on the expression of programmed cell death protein 1 (PD-1) and its ligand (PD-L1) on T and B lymphocytes in patients suffering from recurrent HHV-1 reactivation. We also assessed the effector function of peripheral blood mononuclear cells (PBMCs) after stimulation with thymic peptides. We enrolled 50 women with reactivated HHV-1 infections and healthy volunteers. We measured the expression of various activation and exhaustion markers on the surface of PBMCs using flow cytometry. In ex vivo experiments, we measured the secretion of inflammatory cytokines by PBMCs cultured with thymostimulin. Compared with controls, patients with reactivated HHV-1 infections had increased percentages of CD3+ co-expressing CD25, an activation marker (p< 0.001). Moreover, these patients had increased percentages of CD4+ and CD8+ cells co-expressing the inhibitory markers PD-1 and PD-L1. In cultures of PBMCs from the patients, thymostimulin increased the secretion of interferon gamma (p< 0.001) and interleukin (IL)-2 (p=0.023), but not IL-4 or IL-10.Two-month thymostimulin therapy resulted in no reactivation of HHV-1 infection during this period and the reduction of PD-1 and PD-L1 expression on the surface of T and B lymphocytes (p< 0.001). In conclusion, reactivation of herpes infection is associated with immune exhaustion, which could be reversed by treatment with thymic peptides. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3f2ff13be6d339bebddddfc5356fdc4Test http://europepmc.org/articles/PMC7178259Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a3f2ff13be6d339bebddddfc5356fdc4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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