Time-Resolved Fluoroimmunoassay as an Advantageous Approach for Highly Efficient Determination of Sulfonamides in Environmental Waters
| العنوان: | Time-Resolved Fluoroimmunoassay as an Advantageous Approach for Highly Efficient Determination of Sulfonamides in Environmental Waters |
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| المؤلفون: | Zhen Zhang, Guibin Jiang, Jingfu Liu, Bing Shao |
| المصدر: | Environmental Science & Technology. 44:1030-1035 |
| بيانات النشر: | American Chemical Society (ACS), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Detection limit, Sulfonamides, Time Factors, Chromatography, medicine.diagnostic_test, Sulfadimidine, Metal ions in aqueous solution, Fluoroimmunoassay, Antibodies, Monoclonal, Water, chemistry.chemical_element, General Chemistry, Sulfadiazine, Europium, chemistry, Immunoassay, medicine, Environmental Chemistry, Humic acid, Chelation, Water Pollutants, Chemical, Arsenic, Environmental Monitoring, medicine.drug |
| الوصف: | Using monoclonal antibodies labeled with Eu(3+) chelates, time-resolved fluoroimmunoassay (TRFIA) methods were developed for the determination of trace sulfamethazine (SMZ), sulfa-methoxazole (SMX), and sulfadiazine (SDZ) in environmental waters. Under the optimized conditions, the developed methods offered (i) low detection limits (9.8 ng/L SMZ, 6.1 ng/L SMX, and 5.4 ng/L SDZ, based on 90% inhibition) which were about 1 order of magnitude lower than that of the enzyme-linked immunosorbent assay (ELISA), (ii) high selectivity with no cross-reactivity (0.05%) to similarly structured sulfonamides; (iii) high tolerance to variation of the sample pH (6.0-9.0) and salinity (0-100 mM), as well as the presence of humic acid (0-100 mg/L DOC) and heavy metals (0-1 mg/L concentration each of Cu(2+), Cd(2+), Hg(2+), Pb(2+), and As(V)) in the samples, and (iv) direct determination with low cost, high sample throughput, and low sample consumption (50-100 microL). The proposed TRFIA procedures were applied to determine sulfonamides in a variety of surface water and wastewater samples without sample pretreatment other than filtration. The satisfactory recoveries (64-127%) and reproducibilities (CV = 0.2-16%) achieved, as well as the good agreement with those given by liquid chromatography-tandem mass spectroscopy and ELISA methods, demonstrated the applicability of the proposed TRFIA methods for routine screening/quantification of sulfonamides in environmental waters. |
| تدمد: | 1520-5851 0013-936X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3b8222ea1b7947a95d7bddc22340018Test https://doi.org/10.1021/es903328bTest |
| رقم الانضمام: | edsair.doi.dedup.....a3b8222ea1b7947a95d7bddc22340018 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205851 0013936X |
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