Differential expression and prognostic relevance of autophagy-related markers ATG4B, GABARAP, and LC3B in breast cancer
| العنوان: | Differential expression and prognostic relevance of autophagy-related markers ATG4B, GABARAP, and LC3B in breast cancer |
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| المؤلفون: | Samuel Leung, Samantha Burugu, Karen A. Gelmon, Jamie Magrill, Torsten O. Nielsen, Stephen Yip, Fatemeh Derakhshan, Svetlana Bortnik, Sharon M. Gorski, Basile Tessier-Cloutier, Karama Asleh, Kendall Greening, Tony Ng, Jing Xu |
| المصدر: | Breast Cancer Research and Treatment. 183:525-547 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, Cancer Research, medicine.medical_specialty, GABARAP, Autophagy-Related Proteins, Breast Neoplasms, Kaplan-Meier Estimate, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Internal medicine, Autophagy, Biomarkers, Tumor, Humans, Medicine, Differential expression, Tissue microarray, business.industry, Prognosis, medicine.disease, Immunohistochemistry, Phenotype, 3. Good health, Cysteine Endopeptidases, 030104 developmental biology, 030220 oncology & carcinogenesis, Biomarker (medicine), Female, Apoptosis Regulatory Proteins, business, Microtubule-Associated Proteins |
| الوصف: | Previous studies indicate that breast cancer molecular subtypes differ with respect to their dependency on autophagy, but our knowledge of the differential expression and prognostic significance of autophagy-related biomarkers in breast cancer is limited. Immunohistochemistry (IHC) was performed on tissue microarrays from a large population of 3992 breast cancer patients divided into training and validation cohorts. Consensus staining scores were used to evaluate the expression levels of autophagy proteins LC3B, ATG4B, and GABARAP and determine the associations with clinicopathological variables and molecular biomarkers. Survival analyses were performed using the Kaplan–Meier function and Cox proportional hazards regression models. We found subtype-specific expression differences for ATG4B, with its expression lowest in basal-like breast cancer and highest in Luminal A, but there were no significant associations with patient prognosis. LC3B and GABARAP levels were highest in basal-like breast cancers, and high levels were associated with worse outcomes across all subtypes (DSS; GABARAP: HR 1.43, LC3B puncta: HR 1.43). High ATG4B levels were associated with ER, PR, and BCL2 positivity, while high LC3B and GABARAP levels were associated with ER, PR, and BCL2 negativity, as well as EGFR, HER2, HER3, CA-IX, PD-L1 positivity, and high Ki67 index (p |
| تدمد: | 1573-7217 0167-6806 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e678ae8df71d0c406630dcac94e38b3Test https://doi.org/10.1007/s10549-020-05795-zTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9e678ae8df71d0c406630dcac94e38b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15737217 01676806 |
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