Costunolide attenuates oxygen-glucose deprivation/reperfusion-induced mitochondrial-mediated apoptosis in PC12 cells
| العنوان: | Costunolide attenuates oxygen-glucose deprivation/reperfusion-induced mitochondrial-mediated apoptosis in PC12 cells |
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| المؤلفون: | Huixia Ma, Yafei Zhu, Lanqing Meng, Ting-ting Li, Jinni Meng, Qipeng Zhao |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | D.A. Spandidos, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, caspase, Cell, Biochemistry, Flow cytometry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Western blot, Lactate dehydrogenase, Genetics, medicine, ischemic stroke, Animals, Viability assay, Cytotoxicity, costunolide, Molecular Biology, Membrane Potential, Mitochondrial, medicine.diagnostic_test, L-Lactate Dehydrogenase, Chemistry, apoptosis, PC12 cells, Articles, Molecular biology, oxygen-glucose deprivation/reperfusion, Cell Hypoxia, Mitochondria, Rats, 030104 developmental biology, medicine.anatomical_structure, Glucose, Neuroprotective Agents, Oncology, Apoptosis, 030220 oncology & carcinogenesis, Caspases, Molecular Medicine, Calcium, Sesquiterpenes, Intracellular |
| الوصف: | The present study investigated the effect of costunolide (CT), a compound extracted from Aucklandia lappa Decne, to attenuate oxygen‑glucose deprivation/reperfusion (OGD/R)‑induced mitochondrial‑mediated apoptosis in PC12 cells. The present study used molecular docking technology to detect the binding of CT with mitochondrial apoptotic protein targets. A model of oxygen‑glucose deprivation for 2 h and reperfusion for 24 h in PC12 cells was used to mimic cerebral ischemic injury. Cell viability and damage were measured using the Cell Counting kit‑8 and lactate dehydrogenase (LDH) cytotoxicity assay kits. Cellular apoptosis was analyzed using flow cytometry. A fluorescence microscope determined intracellular [Ca2+] and mitochondrial membrane potential. Furthermore, immunofluorescence and Western blot analyses were used to detect the expression of apoptosis‑associated proteins. CT contains binding sites with Caspase‑3, Caspase‑9 and Caspase‑7. CT markedly enhanced cell viability, inhibited LDH leakage, increased intracellular [Ca2+], stabilized the mitochondrial membrane potential, increased the expression of Bcl‑2 and inhibited the expression of Apaf‑1, Bax, cleaved‑caspase‑7, cleaved‑caspase‑9 and cleaved‑caspase‑3. CT may markedly protect PC12 cells from damage caused by OGD/R, and its mechanism is associated with blocking the calcium channel and inhibiting mitochondrial‑mediated apoptosis. |
| اللغة: | English |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b3347af85d71fff712bb6b332a8c1c8Test http://europepmc.org/articles/PMC8025489Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9b3347af85d71fff712bb6b332a8c1c8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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